Recombinant Mouse FGFR3 alpha (IIIb) Fc Chimera Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 9089-FR

R&D Systems, part of Bio-Techne
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9089-FR-050
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Key Product Details

Source

NS0

Accession #

NP_001156689

Structure / Form

Disulfide-linked homodimer

Conjugate

Unconjugated

Applications

Bioactivity

View all FGFR3 Proteins and Enzymes »

Product Specifications

Source

Mouse myeloma cell line, NS0-derived mouse FGF R3 protein
Mouse FGF R3 (IIIb)
(Ala33-Tyr369)
Accession # NP_001156689		 IEGRMD Human IgG1
(Pro100-Lys330)
N-terminus C-terminus

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Ala33

Predicted Molecular Mass

63 kDa

SDS-PAGE

83-101 kDa, reducing conditions

Activity

Measured by its ability to inhibit FGF acidic-dependent proliferation of BaF3 mouse pro-B cells transfected with human FGF R3 (IIIc).
The ED50 for this effect is 0.2-1.2 μg/mL.

Scientific Data Images for Recombinant Mouse FGFR3 alpha (IIIb) Fc Chimera Protein, CF

Recombinant Mouse FGFR3 alpha (IIIb) Fc Chimera Protein Bioactivity

Recombinant Mouse FGFR3 alpha (IIIb) Fc Chimera Protein Bioactivity

Recombinant Mouse FGF R3 (IIIb) Fc Chimera (Catalog # 9089-FR) inhibits Recombinant Mouse FGF acidic (Catalog # 4686-FA)-dependent proliferation of Human FGF R3 (IIIc) transfected BaF3 mouse pro‑B cells. The ED50 for this effect is 0.2-1.2 μg/mL.

Formulation, Preparation and Storage

9089-FR
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution
Reconstitute at 500 μg/mL in PBS.

Reconstitution Buffer Available:
Size / Price
Qty
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: FGFR3

Fibroblast growth factor receptor 3 (FGF R3), also known as CEK2 and CD333, is an approximately 120 kDa transmembrane receptor tyrosine kinase that plays a role in skeletal development and tumorigenesis (1). Mature mouse FGF R3 (IIIc) consists of a 349 amino acid (aa) extracellular domain (ECD) with three Ig-like domains, a 21 aa transmembrane segment, and a 411 aa cytoplasmic domain that contains the tyrosine kinase domain (2). Alternative splicing generates an additional isoform (IIIb) with a substitution in the third Ig-like domain (3). Within the ECD, mouse FGF R3 (IIIb) shares 91% and 98% aa sequence identity with comparable isoforms of human and rat FGF R3, respectively. The FGF R3 (IIIb) triggers cell proliferation in response to FGF acidic and FGF-9, while FGF R3 (IIIc) shows a wider selectivity that includes FGF acidic, FGF basic, FGF-4, -8, -9, -17, -18, -19, and -20 (4, 5). Ligand binding induces receptor dimerization and acitvation of the tyrosine kinase domain (1). FGF mediated activation of FGF R3 is dependent on the presence of heparan sulfate proteoglycans (6). FGF R3 functions as a negative regulator of endochondral bone growth, and FGF R3 mutations are associated with chondrodysplasia in humans (7, 8). In addition, the development of many cancers is associated with mutations or dysregulation of FGF R3 which can result in constitutive receptor activation (1).

References

  1. Turner, N. and R. Grose (2010) Nat. Rev. Cancer 10:116.
  2. Katoh, O. et al. (1993) Cancer Res. 53:1136.
  3. Chellaiah, A.T. et al. (1994) J. Biol. Chem. 269:11620.
  4. Ornitz, D.M. et al. (1996) J. Biol. Chem. 271:15292.
  5. Zhang, X. et al. (2006) J. Biol. Chem. 281:15694.
  6. Ornitz, D.M. and P. Leder (1992) J. Biol. Chem. 267:16305.
  7. Deng, C. et al. (1996) Cell 84:911.
  8. Colvin, J.S. et al. (1996) Nat. Genet. 12:390.

Long Name

Fibroblast Growth Factor Receptor 3

Alternate Names

CD333, CEK, FGF R3, JTK4

Entrez Gene IDs

2261 (Human); 14184 (Mouse)

Gene Symbol

FGFR3

UniProt

NP_001156689

Additional FGFR3 Products

Product Documents for Recombinant Mouse FGFR3 alpha (IIIb) Fc Chimera Protein, CF

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