Recombinant Mouse Integrin alpha 5 beta 1 Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 7728-A5

R&D Systems, part of Bio-Techne
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7728-A5-050
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Key Product Details

Source

CHO

Accession #

P11688 (Integrin alpha5) & P09055 (Integrin beta1)

Structure / Form

Noncovalently-linked heterodimer 

Conjugate

Unconjugated

Applications

Bioactivity

View all Integrin alpha 5 beta 1 Proteins and Enzymes »

Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived mouse Integrin alpha 5 beta 1 protein
Mouse Integrin alpha5
Phe45-Asn999 (Gly377Ala)
Accession # P11688		 GS Linker Acidic Tail HHHHHH
Mouse Integrin beta1
(Gln21-Asp728)
Accession # P09055		 HP GS Linker Basic Tail
N-terminus C-terminus

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Phe45 (Integrin alpha5) & Gln21 predicted, No results obtained: sequencing might be blocked (Integrin beta1)

Predicted Molecular Mass

113 kDa (Integrin alpha5) & 86.5 kDa (Integrin beta1)

SDS-PAGE

115-165 kDa, reducing conditions

Activity

Measured by its binding ability in a functional ELISA.
When Bovine Fibronectin (Catalog # 1030-FN) is coated at 2 μg/mL, Recombinant Mouse Integrin alpha5 beta1 binds with an apparent KD <0.5 nM. 

Reviewed Applications

Read 1 review rated 4 using 7728-A5 in the following applications:

Formulation, Preparation and Storage

7728-A5
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution
Reconstitute at 500 μg/mL in PBS.

Reconstitution Buffer Available:
Size / Price
Qty
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Integrin alpha 5 beta 1

Integrin alpha 5 beta 1, also known as VLA‑5, is a widely expressed non‑covalent heterodimer of a 160 kDa alpha5 and a 130 kDa beta1 Integrin subunit. alpha5/ beta1 functions in cell adhesion, migration, activation, and matrix fibrillogenesis (1, 2). Mature mouse Integrin  alpha5/CD49e consists of a 955 aa extracellular domain (ECD) with 7 tandem FG‑GAP repeats, a 26 aa transmembrane segment, and a 28 aa cytoplasmic domain (3). Within the ECD, mouse alpha5 shares 91% and 97% aa sequence identity with human and rat alpha5, respectively. Mature mouse Integrin beta1/CD29 consists of a 708 aa ECD with one vWF‑A domain and a cysteine‑rich region, followed by a 23 aa transmembrane segment and a 47 aa cytoplasmic domain (3). Within the ECD, mouse beta1 shares 93% and 98% aa sequence identity with human and rat beta1, respectively. alpha5/ beta1 binds Fibronectin in both RGD‑dependent and ‑independent manners (4, 5). It is up‑regulated on tumor vasculature and promotes angiogenesis (6, 7). This is accomplished in part by a constitutive association in cis of alpha5/ beta1 with VEGF R3 and Tie‑2, a requirement for optimal activation of those receptors (8, 9). alpha5/ beta1 interacts with a variety of other proteins, including ErbB2, uPAR, Galectin‑1, CTGF, and thrombin‑cleaved Osteopontin (5, 10‑13). alpha5/ beta1 also functions on some hematopoietic and neuronal stem cells (14‑16).

References

  1. Mao, Y. and Schwarzbauer, J.E. (2005) Matrix Biol. 24:389.
  2. Gu, J. and Taniguchi, N. (2004) Glycoconj. J. 21:9.
  3. Holers, V.M. et al. (1989) J. Exp. Med. 169:1589.
  4. Takagi, J. et al. (2003) EMBO J. 22:4607.
  5. Wei, Y. et al. (2005) J. Cell Biol. 168:501.
  6. Parsons-Wingerter, P. et al. (2005) Am. J. Pathol. 167:193.
  7. Kim, S. et al. (2000) Am. J. Pathol. 156:1345.
  8. Zhang, X. et al. (2004) J. Cell. Physiol. 202:205.
  9. Cascone, I. et al. (2005) J. Cell Biol. 170:993.
  10. Kuwada, S.K. et al. (2005) J. Biol. Chem. 280:19027.
  11. Fischer, C. et al. (2005) J. Biol. Chem. 280:37266.
  12. Gao, R. and Brigstock, D.R. (2005) Gastroenterology 129:1019.
  13. Yokosaki, Y. et al. (2005) Matrix Biol. 24:418.
  14. Carstanjen, D. et al. (2005) Transfusion 45:1192.
  15. Gibson, R.M. et al. (2005) Mol. Cell. Neurosci. 28:588.
  16. Tanaka, R. et al. (2009) J. Biol. Chem. 284:19817.

Alternate Names

VLA-5

Entrez Gene IDs

3678 (Human)

Gene Symbol

ITGA5

UniProt

P11688 (Integrin alpha5) & P09055 (Integrin beta1)

Additional Integrin alpha 5 beta 1 Products

Product Documents for Recombinant Mouse Integrin alpha 5 beta 1 Protein, CF

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Product Specific Notices for Recombinant Mouse Integrin alpha 5 beta 1 Protein, CF

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