Recombinant Mouse MuSK His-tag Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 11519-MK

R&D Systems, part of Bio-Techne
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11519-MK-050
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Key Product Details

Source

HEK293

Accession #

Q61006.1

Conjugate

Unconjugated

Applications

Bioactivity

View all MuSK Proteins and Enzymes »

Product Specifications

Source

Human embryonic kidney cell, HEK293-derived mouse MuSK protein
Glu22-Thr494, with a C-terminal 6-His tag

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Glu22

Predicted Molecular Mass

53 kDa

SDS-PAGE

63-69 kDa, under reducing conditions.

Activity

Measured by its binding ability in a functional ELISA.
Recombinant Mouse MuSK His-tag binds to Recombinant Mouse LRP-4 His-tag (Catalog # 10229-LR) and Agrin His-tag with a ED50 of 0.0800-0.800 μg/mL.

Scientific Data Images for Recombinant Mouse MuSK His-tag Protein, CF

Recombinant Mouse MuSK His-tag Protein Binding Activity.

Recombinant Mouse MuSK His-tag Protein (Catalog # 11519-MK) binds to Recombinant Mouse LRP-4 His-tag Protein (10229-LR) and Agrin His-tag Protein with an ED50 of 0.0800-0.800 μg/mL.

Recombinant Mouse MuSK His-tag Protein SDS-PAGE.

2 μg/lane of Recombinant Mouse MuSK His-tag Protein (Catalog # 11519-MK) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 63 - 69 kDa.

Formulation, Preparation and Storage

11519-MK
Formulation Supplied as a 0.2 μm filtered solution in PBS with Trehalose.
Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after opening.
  • 3 months, -20 to -70 °C under sterile conditions after opening.

Background: MuSK

MuSK (muscle-specific kinase) is a 100-kDa type I transmembrane (TM) protein belonging to the receptor tyrosine kinase family (1). It is found in the postsynaptic membrane of skeletal muscle motor endplates (2). MuSK contains a 473 aa extracellular domain (ECD), a 21 aa transmembrane domain, and a 353 cytoplasmic domain. Human MuSK has multiple isoforms. One contains deletions at residues 307-394 and 454-461, while a second is a short soluble form that contains residues 120­-209 plus a unique 24 aa C­terminal tail (3). Within the ECD, mouse MuSK shares 91% and 96% aa sequence identity with human and rat MuSK, respectively. MuSK is a crucial signaling molecule in the formation of the neuromuscular junction(NMJ). Defects in MuSK causes muscle weakness in congenital myathenic syndromes (4). MuSK binds the heparin sulfate proteoglycan agrin to promote acetylcholine receptor clustering. It has also been found to bind with low-density lipoprotein receptor-related protein 4 (LRP4) (5), Wnt ligands (6), Biglycan (7), ColQ (8) and BMPs(10). Recent studies have showed that MuSK does not bind agrin directly, but enhanced the MuSK-LRP4 interaction (8, 9). When agrin binds to the N-terminal region of LRP4, this promotes the association of LRP4 and MuSK, which then stimulates MuSK kinase activity (11, 12).

References

  1. Valenzuela, D.M. et al. (1995) Neuron 15:573.
  2. Finn, A.J. et al. (2003) Nat. Neurosci. 6:717.
  3. Nasrin, F. et al. (2014) Sci. Rep. 4:6841.
  4. DeChiara, T. et al. (1996) Cell 85:501.
  5. Kim, N. et al. (2008) Cell 135:334.
  6. Banerjee, S. et al. (2011) Development 138:3287.
  7. Amenta, A.R. et al. (2012) J. Neurosci. 32:2324.
  8. Otsuka, K. et al. (2015) Scientific Reports 5:13928.
  9. Zhang, B. et al. (2008) Neuron 60:285.
  10. Yilmaz, A. et al. (2016) Science Signaling 9:ra87.
  11. Zhang, W. et al. (2011) J. Biol. Chem. 286:40624.
  12. Burden, S.J. et al. (2017) CSH Perspectives Biol. 5:a009167.

Long Name

Muscle-specific Receptor Tyrosine Kinase

Alternate Names

EC 2.7.10, EC 2.7.10.1, MGC126323, MGC126324, muscle, skeletal, receptor tyrosine kinase, MuSK, skeletal receptor tyrosine-protein kinase

Entrez Gene IDs

4593 (Human); 18198 (Mouse); 81725 (Rat); 102127677 (Cynomolgus Monkey)

Gene Symbol

MUSK

UniProt

Q61006.1

Additional MuSK Products

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Product Specific Notices for Recombinant Mouse MuSK His-tag Protein, CF

For research use only

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