Recombinant Mouse TACE/ADAM17 Protein, CF
R&D Systems, part of Bio-Techne | Catalog # 2978-AD
Key Product Details
Source
NS0
Accession #
Structure / Form
Recombinant Mouse TACE/ADAM17 is prone to proteolytic cleavage at C-terminus.
The poly-His
tag may not be present in the preparation.
Conjugate
Unconjugated
Applications
Enzyme Activity
Product Specifications
Source
Mouse myeloma cell line, NS0-derived mouse TACE/ADAM17 protein
Pro18-Asp563 and Arg215-Asp563, both with a C-terminal 10-His tag, Pro18-Arg214 and Asp59-Arg214
Pro18-Asp563 and Arg215-Asp563, both with a C-terminal 10-His tag, Pro18-Arg214 and Asp59-Arg214
Purity
>90%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Level
<1.0 EU per 1 μg of the protein by the LAL method.
N-terminal Sequence Analysis
Pro18, Asp59 & Arg215
Predicted Molecular Mass
63 kDa, 40 kDa, 23 kDa, 18 kDa
SDS-PAGE
76 kDa, 63 kDa, 21 kDa and 18 kDa, reducing conditions
Activity
Measured by its ability to cleave a fluorogenic peptide substrate Mca-PLAQAV-Dpa-RSSSR-NH2 (Catalog # ES003).
The specific activity is >500 pmol/min/µg, as measured under the described conditions. Note: It is extremely important that the assay solution does not contain salt as it inhibits TACE activity.
The specific activity is >500 pmol/min/µg, as measured under the described conditions. Note: It is extremely important that the assay solution does not contain salt as it inhibits TACE activity.
Formulation, Preparation and Storage
2978-AD
| Formulation | Lyophilized from a 0.2 μm filtered solution in MES and NaCl. |
| Reconstitution |
Reconstitute at 100 μg/mL in sterile, deionized water.
|
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Background: TACE/ADAM17
Tumor necrosis factor alpha (TNF-alpha) converting enzyme (TACE) is a member of the ADAM family that contains a disintegrin and metalloprotease-like domain. The domain structure of mouse TACE consists of pro (aa 18-214), catalytic (aa 215-474), disintegrin-like (aa 475-563), cysteine-rich (aa 564-602), Crambin-like (aa 603-671), transmembrane (aa 672-692), and cytoplasmic (aa 693-827) (1, 2). Recombinant mouse TACE ends after the disintegrin-like domain and consists of several forms as described. In addition to its ability to release the 17 kDa extracellular form of TNF-alpha from the 26 kDa membrane-anchored TNF-alpha, TACE also plays an essential role in shedding ectodomains from a variety of proteins such as L-selectin, transforming growth factor-alpha, amyloid protein precursor, and Notch1 receptor. TACE mRNA is present in virtually every tissue and TACE protein resides both on the cell surface and in the cell (3).
References
- Cerretti, D.P. et al. (1999) Cytokine 11:541.
- Mizui, Y. et al. (1999) Gene 233:67.
- Black, R.A. (2004) Handbook of Proteolytic Enzymes, Barrett, A.J. et al. eds. pp. 726, Academic Press, San Diego.
Long Name
TNF-alpha Converting Enzyme
Alternate Names
ADAM17, CD156b
Gene Symbol
ADAM17
UniProt
Additional TACE/ADAM17 Products
Product Documents for Recombinant Mouse TACE/ADAM17 Protein, CF
Product Specific Notices for Recombinant Mouse TACE/ADAM17 Protein, CF
For research use only
Loading...
Loading...
Loading...
Loading...