Recombinant Mouse TIM-1/KIM-1/HAVCR Protein
R&D Systems, part of Bio-Techne | Catalog # 1817-TM
This protein is in the process of being discontinued.
Key Product Details
Product Specifications
Source
Mouse myeloma cell line, NS0-derived mouse TIM-1/KIM-1/HAVCR protein
Tyr22-Thr212, with a C-terminal 6-His tag
Tyr22-Thr212, with a C-terminal 6-His tag
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
N-terminal Sequence Analysis
Tyr22
Predicted Molecular Mass
21.8 kDa
SDS-PAGE
40-50 kDa, reducing conditions
Activity
Measured by its ability to induce IL-6 or TNF-alpha secretion by RAW 264.7 mouse monocyte/macrophage cells.
The ED50 for this effect is 0.1-0.4 µg/mL.
Measured by its ability to bind THP-1 human acute monocytic leukemia cells.
As determined by flow cytometric analysis, there is a greater than 3 fold increase in the fluorescence of apoptotic THP-1 cells treated with 0.5 μg/mL recombinant mouse TIM-1/His and Mouse Anti-polyHistidine PE-conjugated Monoclonal Antibody (Catalog # IC050P) compared to cells stained with anti‑Mouse Anti‑polyHistidine PE-conjugated Monoclonal Antibody alone.
The ED50 for this effect is 0.1-0.4 µg/mL.
Measured by its ability to bind THP-1 human acute monocytic leukemia cells.
As determined by flow cytometric analysis, there is a greater than 3 fold increase in the fluorescence of apoptotic THP-1 cells treated with 0.5 μg/mL recombinant mouse TIM-1/His and Mouse Anti-polyHistidine PE-conjugated Monoclonal Antibody (Catalog # IC050P) compared to cells stained with anti‑Mouse Anti‑polyHistidine PE-conjugated Monoclonal Antibody alone.
Formulation, Preparation and Storage
Carrier Free
What does CF mean?CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
What formulation is right for me?In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
Carrier: 1817-TM
| Formulation | Lyophilized from a 0.2 μm filtered solution in Tris-Citrate and NaCl with BSA as a carrier protein. |
| Reconstitution | Reconstitute at 100 μg/mL in sterile PBS. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Carrier Free: 1817-TM/CF
| Formulation | Lyophilized from a 0.2 μm filtered solution in Tris-Citrate and NaCl. |
| Reconstitution | Reconstitute at 100 μg/mL in sterile PBS. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Background: TIM-1/KIM-1/HAVCR
TIM‑1 (T cell‑immunoglobulin‑mucin; also KIM‑1 and Tapr) is a 70 ‑ 80 kDa, type I transmembrane glycoprotein member of the TIM family of immunoglobulin superfamily molecules (1, 2, 3, 4). This gene family is involved in the regulation of Th1 and Th2‑cell‑mediated immunity. In mouse, there are eight known TIM genes (# 1 ‑ 8) vs. only three genes in human (# 1, 3 & 4) (1, 2). Mouse TIM‑1 and ‑2 are counterparts of human TIM‑1, while mouse TIM‑5 through TIM‑8 have no human counterparts (2). Mouse TIM‑1 (isoform 2) is synthesized as a 282 amino acid (aa) precursor that contains a 21 aa signal sequence, a 193 aa extracellular domain (ECD), a 21 aa transmembrane segment and a 47 aa cytoplasmic domain (5, 6). The ECD contains one V‑type Ig‑like domain and a mucin region characterized by multiple T‑S‑P motifs. The mucin region undergoes extensive O‑linked glycosylation. The mouse TIM‑1 gene is highly polymorphic and, based on rat, may undergo alternate splicing (4, 6). One isoform (termed isoform 1) possesses a 23 aa insertion after Pro182 (GenBank # NP_599099). Another splice variant (of isoform 1) shows a 15 aa deletion in the mucin region of the ECD (6). This difference is associated with a decreased susceptibility to asthma. In human, TIM‑1 is known to circulate as a soluble form that arises from cleavage by an undefined MMP, releasing an 85 ‑ 90 kDa soluble molecule (7). In mouse, a 60 ‑ 65 kDa soluble form has also been detected (in urine) that presumably arises from proteolytic processing (8). In‑house data from R&D Systems Inc. has demonstrated the presence of soluble TIM‑1 in mouse circulation. The ECD of mouse TIM‑1 shares 37% and 81% aa sequence identity with human and rat TIM‑1 ECD, respectively. There are at least three reported ligands for TIM‑1, and include TIM‑4, phosphatidlyserine and the hepatitis A virus (3, 9, 10). However, still others are believed to exist, and based on the ligand for TIM‑3, one might be an S‑type lectin (11). TIM‑1 ligation induces T cell proliferation and promotes cytokine production (1, 11). In particular, it induces IL‑4 production, and requires the TIM‑1 cytoplasmic tyrosine phosphorylation motif (5).
References
- Meyers, J.H. et al. (2005) Trends Mol. Med. 11:1471.
- Su, E.W. et al. (2008) Cytokine 44:9.
- Freeman, G.J. et al. (2010) Immunol. Rev. 235:172.
- Ichimura, T. et al. (1998) J. Biol. Chem. 273:4135.
- de Souza, A.J. et al. (2005) Proc. Natl. Acad. Sci. USA 102:17113.
- McIntire, J.J. et al. (2001) Nat. Immunol. 2:1109.
- Bailly, V. et al. (2002) J. Biol. Chem. 277:39739.
- Herzog, C. et al. (2007) Kidney Int. 71:1009.
- Feigelstock, D. et al. (1998) J. Virol. 72:6621.
- Zhu, C. et al. (2005) Nat. Immunol. 6:1245.
- Meyers, J.H. et al. (2005) Nat. Immunol. 6:455.
Long Name
T Cell Immunoglobulin Mucin-1
Alternate Names
CD365, HAVCR1, KIM-1, TIM1
Gene Symbol
HAVCR1
UniProt
Additional TIM-1/KIM-1/HAVCR Products
Product Documents for Recombinant Mouse TIM-1/KIM-1/HAVCR Protein
Product Specific Notices for Recombinant Mouse TIM-1/KIM-1/HAVCR Protein
For research use only
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