Recombinant Mouse TIMP-2 Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 6304-TM

R&D Systems, part of Bio-Techne
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6304-TM-010
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Key Product Details

Source

NS0

Accession #

NP_035724

Conjugate

Unconjugated

Applications

Enzyme Activity

View all TIMP-2 Proteins and Enzymes »

Product Specifications

Source

Mouse myeloma cell line, NS0-derived mouse TIMP-2 protein
Met1-Pro220

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain at 5 μg per lane.

Endotoxin Level

<1.0 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Cys27

Predicted Molecular Mass

22 kDa

SDS-PAGE

22 kDa, reducing conditions

Activity

Measured by its ability to inhibit human MMP-2 cleavage of a fluorogenic peptide substrate Mca-PLGL-Dpa-AR-NH2 (Catalog # ES001).
The IC50 value is <2 nM, as measured under the described conditions.

Formulation, Preparation and Storage

6304-TM
Formulation Supplied as a 0.2 μm filtered solution in Tris and NaCl.
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -20 to -70 °C as supplied.
  • 3 months, -20 to -70 °C under sterile conditions after opening.

Background: TIMP-2

Tissue inhibitors of metalloproteinases (TIMPs) are a family of proteins that regulate the activation and proteolytic activity of matrix metalloproteinases (MMPs). There are four mammalian members of the family; TIMP‑1, TIMP‑2, TIMP‑3, and TIMP‑4. TIMP‑2 is a non‑glycosylated protein of molecular mass 22 kDa that is secreted by a wide range of cell types that inhibits MMPs non‑covalently by the formation of binary complexes. TIMP‑2 interacts with MMP‑14 to facilitate the cell‑surface activation of pro‑MMP‑2 (1). TIMP‑2 has other functions independent of the inhibition of MMPs. The binding of TIMP‑2 to a3b1 integrin at the surface of endothelial cells results in the inhibition of endothelial cell proliferation and angiogenesis (2).

References

  1. Nagase, H. (1998) Cell Res. 81:179.
  2. Stetler-Stevenson, W.G. (2008) Sci. Signal. 1:re6.

Long Name

Tissue Inhibitors of Metalloproteinases 2

Alternate Names

TIMP2

Entrez Gene IDs

7077 (Human); 21858 (Mouse); 29543 (Rat)

Gene Symbol

TIMP2

UniProt

NP_035724

Additional TIMP-2 Products

Product Documents for Recombinant Mouse TIMP-2 Protein, CF

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Recombinant Mouse TIMP-2 Protein, CF

Coomassie is a registered trademark of Imperial Chemical Industries Ltd.

For research use only

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