Recombinant Mouse TLR4 Fc Chimera Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 9149-TR

R&D Systems, part of Bio-Techne
Catalog #
Availability
Size / Price
Qty
9149-TR-050
Print Quote
Bulk Order
100% Guarantee

Key Product Details

Source

CHO

Accession #

Q9QUK6

Structure / Form

Disulfide-linked homodimer

Conjugate

Unconjugated

Applications

Bioactivity

View all TLR4 Proteins and Enzymes »

Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived mouse TLR4 protein
Mouse TLR4
(Asn26-Lys629)
Accession # Q9QUK6		 DIEGRMDP Mouse IgG2a
(Glu98-Lys330)
N-terminus C-terminus

Purity

>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Asn26

Predicted Molecular Mass

96 kDa

SDS-PAGE

115-137 kDa, reducing conditions

Activity

Measured by its binding ability in a functional ELISA.
When Recombinant Mouse TLR4 Fc Chimera is immobilized at 2 μg/mL, 100 μL/well, the concentration of Recombinant Human MD-2 (Catalog # 1787-MD) that produces 50% of the optimal binding response is approximately 0.15-0.9 μg/mL

Scientific Data Images for Recombinant Mouse TLR4 Fc Chimera Protein, CF

Recombinant Mouse TLR4 Fc Chimera Protein Bioactivity

Recombinant Mouse TLR4 Fc Chimera Protein Bioactivity

When Recombinant Mouse TLR4 Fc Chimera (Catalog # 9149-TR) is coated at 2 µg/mL (100 µL/well), Recombinant Human MD-2 (Catalog # 1787-MD) binds with a typical ED50 of 0.15-0.9 µg/mL.

Formulation, Preparation and Storage

9149-TR
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution
Reconstitute at 500 μg/mL in PBS.

Reconstitution Buffer Available:
Size / Price
Qty
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TLR4

TLR4, also known as CD284, is a 100 kDa type I transmembrane glycoprotein that belongs to the mammalian Toll-Like Receptor family of pathogen pattern recognition molecules. The complex of TLR4 with MD-2 functions as a critical receptor for bacterial endotoxin/lipopolysaccharide (LPS) (1-3). Mature mouse TLR4 consists of a 613 amino acid (aa) extracellular domain (ECD), a 21 aa transmembrane segment, and a 176 aa cytoplasmic domain. TLR4 contains 19 leucine rich repeats in its ECD and one cytoplasmic Toll/IL-1 receptor (TIR) domain (4-6). Within the ECD, mouse TLR4 shares 63% and 83% aa sequence identity with human and rat TLR4, respectively. On monocytes, macrophages, dendritic cells, and B cells, MD‑2 expression is required for cell surface localization of TLR4 and for optimal LPS‑induced TLR4 signaling (7-10). MD‑2 also forms soluble disulfide-linked homo-oligomers which can interact with TLR4 (8). Through a domain separate from its TLR4-binding domain, MD‑2 extracts LPS from circulating CD14‑LPS complexes and carries the LPS into a ternary complex with TLR4 (11-13). The interaction of
MD‑2/LPS with TLR4 induces receptor oligomerization and the triggering of an inflammatory response (2, 14). Increased levels of plasma MD‑2 in septic shock patients sensitizes MD‑2 non-expressing epithelial cells to LPS and promotes widespread tissue inflammation (15).

References

  1. Mukherjee, S. et al. (2016) Braz. J. Infect. Dis. 20:193.
  2. Plociennikowska, A. et al. (2015) Cell. Mol. Life Sci. 72:557.
  3. Erridge, C. (2010) J. Leukoc. Biol. 87:989.
  4. Medzhitov, R. et al. (1997) Nature 388:394.
  5. Poltorak, A. et al. (1998) Science 282:2085.
  6. Qureshi, S.T. et al. (1999) J. Exp. Med. 189:615.
  7. Shimazu, R. et al. (1999) J. Exp. Med. 189:1777.
  8. Visintin, A. et al. (2001) Proc. Natl. Acad. Sci. USA 98:12156.
  9. Akashi, S. et al. (2000) J. Immunol. 164:3471.
  10. Nagai, Y. et al. (2002) Nat. Immunol. 3:667.
  11. Re, F. and J.L. Strominger (2003) J. Immunol. 171:5272.
  12. Kennedy, M.N. et al. (2004) J. Biol. Chem. 279:34698.
  13. Gioannini, T.L. et al. (2004) Proc. Natl. Acad. Sci. USA 101:4186.
  14. Saitoh, S. et al. (2004) J. Endotoxin Res. 10:257.
  15. Pugin, J. et al. (2004) Blood 104:4071.

Long Name

Toll-like Receptor 4

Alternate Names

CD284

Entrez Gene IDs

7099 (Human); 21898 (Mouse); 29260 (Rat)

Gene Symbol

TLR4

UniProt

Q9QUK6

Additional TLR4 Products

Product Documents for Recombinant Mouse TLR4 Fc Chimera Protein, CF

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Recombinant Mouse TLR4 Fc Chimera Protein, CF

For research use only

Privacy and Cookie Policy
Site Map
© Copyright 2024 Bio-Techne. All Rights Reserved.