Recombinant Rat GITR Ligand/TNFSF18 Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 9735-GL

R&D Systems, part of Bio-Techne
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9735-GL-050
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Key Product Details

Source

NS0

Accession #

XP_008767927

Conjugate

Unconjugated

Applications

Bioactivity

View all GITR Ligand/TNFSF18 Proteins and Enzymes »

Product Specifications

Source

Mouse myeloma cell line, NS0-derived rat GITR Ligand/TNFSF18 protein
Thr74-Ser200, with a N-terminal HA-tag

Purity

>85%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Tyr

Predicted Molecular Mass

16 kDa

SDS-PAGE

19-22 kDa, reducing conditions

Activity

Measured by its ability to induce IL-8 secretion in HT1080 human fibrosarcoma cells transfected with rat GITR.
The ED50 for this effect is 0.06-0.6 μg/mL in the presence of Mouse Anti-Hemagglutinin/HA Peptide Monoclonal Antibody (Catalog # MAB060).

Scientific Data Images for Recombinant Rat GITR Ligand/TNFSF18 Protein, CF

Recombinant Rat GITR Ligand/TNFSF18 Protein Bioactivity

Recombinant Rat GITR Ligand/TNFSF18 Protein Bioactivity

Recombinant Rat GITR Ligand/TNFSF18 (Catalog # 9735-GL) induces IL-8 secretion in HT1080 human fibrosarcoma cells transfected with rat GITR. The ED50 for this effect is 0.06-0.6 µg/mL.

Formulation, Preparation and Storage

9735-GL
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution
Reconstitute at 500 μg/mL in PBS.

Reconstitution Buffer Available:
Size / Price
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Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
  • 12 months from date of receipt, ≤ -20 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, ≤ -20 °C under sterile conditions after reconstitution.

Background: GITR Ligand/TNFSF18

GITR Ligand, also known as TNFSF18 and TL6, is an approximately 30 kDa type II transmembrane glycoprotein in the TNF superfamily (1). Rat GITR Ligand consists of a 20 amino acid (aa) cytoplasmic domain, a 21 aa transmembrane segment, and a 132 aa extracellular domain (ECD) (2-4). Within the ECD, rat GITR Ligand shares 60.6% and 81.1% aa sequence identity with human and mouse GITR Ligand, respectively. GITR Ligand is expressed on antigen presenting cells, CD4-CD8- double negative thymic precursors, vascular endothelial cells, neurons, and in the eye (4-11). Its expression is transiently up-regulated by proinflammatory stimulation (5, 8, 11). The binding of GITR Ligand to GITR on mouse CD25+ T reg cells permits the reactivation of T cells from Treg-induced suppression, although this does not appear to occur in humans (9, 12-14). GITR Ligand binding to GITR additionally provides a co-stimulatory signal to activated CD4+ and CD8+ T cells and NK cells (6, 15, 16). This interaction also induces reverse signaling in GITR Ligand expressing dendritic cells to suppress cellular activation through the same pathway induced by the immunosuppressant dexamethasone (17). In the brain, GITR Ligand/GITR interactions enhance NGF-mediated neurite outgrowth from sympathetic neurons (10).

References

  1. Azuma, M. (2010) Crit. Rev. Immunol. 30:547.
  2. Yu, K.Y. et al. (2003) Biochem. Biophys. Res. Commun. 310:433.
  3. Kim, J.D. et al. (2003) Genes Immun. 4:564.
  4. Tone, M. et al. (2003) Proc. Natl. Acad. Sci. 100:15059.
  5. Stephens, G.L. et al. (2004) J. Immunol. 173:5008.
  6. Hanabuchi, S. et al. (2006) Blood 107:3617.
  7. Kamimura, Y. et al. (2009) J. Immunol. 182:2708.
  8. Hwang, H. et al. (2010) J. Neurosci. Res. 88:2188.
  9. Tuyaerts, S. et al. (2007) J. Leukoc. Biol. 82:93.
  10. O'Keefe, G.W. et al. (2008) Nat. Neurosci. 11:135.
  11. Kim, B.J. et al. (2004) Invest. Ophthalmol. Vis. Sci. 45:3170.
  12. Shimizu, J. et al. (2002) Nat. Immunol. 3:135.
  13. Ji, H. et al. (2004) J. Immunol. 172:5823.
  14. McHugh, R.S. et al. (2002) Immunity 16:311.
  15. Kanamaru, F. et al. (2004) J. Immunol. 172:7306.
  16. Ronchetti, S. et al. (2004) Eur. J. Immunol. 34:613.
  17. Grohmann, U. et al. (2007) Nat. Med. 13:579.

Long Name

Glucocorticoid Induced TNF Receptor Family Related Gene Ligand

Alternate Names

AITRL, GITRL, TNFSF18

Entrez Gene IDs

8995 (Human); 240873 (Mouse); 364031 (Rat)

Gene Symbol

TNFSF18

UniProt

XP_008767927

Additional GITR Ligand/TNFSF18 Products

Product Documents for Recombinant Rat GITR Ligand/TNFSF18 Protein, CF

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Product Specific Notices for Recombinant Rat GITR Ligand/TNFSF18 Protein, CF

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