Recombinant SARS-CoV-2 C.36.3 Spike GCN4-IZ His Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 10886-CV

R&D Systems, part of Bio-Techne
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10886-CV-100
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Key Product Details

Source

HEK293

Accession #

YP_009724390.1

Conjugate

Unconjugated

Applications

Bioactivity

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Product Specifications

Source

Human embryonic kidney cell, HEK293-derived sars-cov-2 Spike protein
SARS-CoV-2 C.36.3 Spike
(Val16-Lys1211)
(His69 del, Val70 del, Trp152Arg, Arg346Ser, Leu452Arg, Asp614Gly, Gln677His, Ala899Ser) (Arg682Ser, Arg685Ser, Lys986Pro, Val987Pro)
Accession # YP_009724390.1		 GCN4-IZ 6-His tag
N-terminus C-terminus

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Val16

Predicted Molecular Mass

138 kDa

SDS-PAGE

142-173 kDa, under reducing conditions.

Activity

Measured by its binding ability in a functional ELISA with Recombinant Human ACE-2 His-tag (Catalog # 933-ZN).

Scientific Data Images for Recombinant SARS-CoV-2 C.36.3 Spike GCN4-IZ His Protein, CF

Recombinant SARS-CoV-2 C.36.3 Spike (GCN4-IZ) His Protein Binding Activity

Recombinant SARS-CoV-2 C.36.3 Spike (GCN4-IZ) His-tag (Catalog # 10886-CV) binds Recombinant Human ACE-2 His-tag (Catalog # 933-ZN) in a functional ELISA.

Recombinant SARS-CoV-2 C.36.3 Spike (GCN4-IZ) His Protein SDS-PAGE.

2 μg/lane of Recombinant SARS-CoV-2 C.36.3 Spike (GCN4-IZ) His Protein (Catalog # 10886-CV) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 142-173 kDa.

Formulation, Preparation and Storage

10886-CV
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Spike

SARS-CoV-2, which causes the global pandemic coronavirus disease 2019 (COVID-19), belongs to a family of viruses known as coronaviruses that also include MERS and SARS-CoV-1. Coronaviruses are commonly comprised of four structural proteins: Spike protein (S), Envelope protein (E), Membrane protein (M) and Nucleocapsid protein (N) (1). The SARS-CoV-2 S protein is a glycoprotein that mediates membrane fusion and viral entry. The S protein is homotrimeric, with each ~180-kDa monomer consisting of two subunits, S1 and S2 (2). In SARS-CoV-2, as with most coronaviruses, proteolytic cleavage of the S protein into S1 and S2 subunits is required for activation. The S1 subunit is focused on attachment of the protein to the host receptor while the S2 subunit is involved with cell fusion (3-5). A metallopeptidase, angiotensin-converting enzyme 2 (ACE-2), has been identified as a functional receptor for SARS-CoV-2 through interaction with a receptor binding domain (RBD) located at the C-terminus of S1 subunit (6, 7). The S protein of SARS-CoV-2 shares 75% and 29% amino acid sequence identity with S protein of SARS‑CoV-1 and MERS, respectively. A SARS-CoV-2 variant (C.36.3) carrying the aa substitution H69 del, V70 del, W152R, R346S, L452R, D614G, Q677H was first identified in Thailand, traveled from Egypt. This variant is one of the Kappa lineage.

References

  1. Wu, F. et al. (2020) Nature 579:265.
  2. Tortorici, M.A. and D. Veesler (2019) Adv. Virus Res. 105:93.
  3. Bosch, B.J. et al. (2003). J. Virol. 77:8801.
  4. Belouzard, S. et al. (2009) Proc. Natl. Acad. Sci. 106:5871.     
  5. Millet, J.K. and G.R. Whittaker (2015) Virus Res. 202:120.     
  6. Li, W. et al. (2003) Nature 426:450.     
  7. Wong, S.K. et al. (2004) J. Biol. Chem. 279:3197.

Long Name

Spike Protein

Alternate Names

S Protein

Entrez Gene IDs

918758 (HCoV-229E); 2943499 (HCoV-NL63); 39105218 (HCoV-OC43); 37616432 (MERS-CoV); 1489668 (SARS-CoV); 43740568 (SARS-CoV-2)

Gene Symbol

S

UniProt

YP_009724390.1

Additional Spike Products

Product Documents for Recombinant SARS-CoV-2 C.36.3 Spike GCN4-IZ His Protein, CF

Product Datasheets

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Product Specific Notices for Recombinant SARS-CoV-2 C.36.3 Spike GCN4-IZ His Protein, CF

For research use only

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