Recombinant Viral 136R/Y136 Protein
R&D Systems, part of Bio-Techne | Catalog # 8976-BR
Type I and III Interferon Inhibitor
Key Product Details
Source
CHO
Conjugate
Unconjugated
Applications
Bioactivity
Product Specifications
Source
Chinese Hamster Ovary cell line, CHO-derived viral 136R protein
| Viral 136R/Y136 (Tyr17-Lys351) Accession # Q9DHH7 |
HPGGGSGGGSGGGS | HHHHHH |
| N-terminus | C-terminus |
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
N-terminal Sequence Analysis
Tyr17
Predicted Molecular Mass
41 kDa
SDS-PAGE
88-120 kDa, reducing conditions
Activity
Measured by its ability to inhibit Type-I and Type-III IFN-mediated anti-viral activity. Huang, J. et al. (2007) PANS 104:9822.
The ED50 for this effect is 2-12 ng/mL in the presence of 20 pg/mL Recombinant Human IFN-beta (Catalog # 8499-IF).
The ED50 for this effect is 2-12 ng/mL in the presence of 20 pg/mL Recombinant Human IFN-beta (Catalog # 8499-IF).
Formulation, Preparation and Storage
Carrier Free
What does CF mean?CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
What formulation is right for me?In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
Carrier: 8976-BR
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein. |
| Reconstitution | Reconstitute at 500 μg/mL in PBS. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Carrier Free: 8976-BR/CF
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. |
| Reconstitution | Reconstitute at 500 μg/mL in PBS. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Background: 136R
References
- Huang, J. et al. (2007) Proc. Natl. Acad. Sci. USA 104:9822.
Long Name
Soluble Type I Interferon Receptor 136R
Additional 136R Products
Product Documents for Recombinant Viral 136R/Y136 Protein
Product Specific Notices for Recombinant Viral 136R/Y136 Protein
For research use only
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