Recombinant Human Smad2 His Protein

Name: Recombinant Human Smad2 His Protein
Brand: Novus Biologicals
SKU: NBC1-25856
Rating: 5 (1 reviews)

Novus Biologicals, part of Bio-Techne | Catalog # NBC1-25856

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Technical Data
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Product Specifications
Scientific Data
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Source

E. coli

Tag

His

Conjugate

Unconjugated

Applications

SDS-PAGE

View all Smad2 Proteins and Enzymes »

Product Specifications

Description

A recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 1- 467 of Human Smad2

Source: E.coli

Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MSSILPFTPP VVKRLLGWKK SAGGSGGAGG GEQNGQEEKW CEKAVKSLVK KLKKTGRLDE LEKAITTQNC NTKCVTIPST CSEIWGLSTP NTIDQWDTTG LYSFSEQTRS LDGRLQVSHR KGLPHVIYCR LWRWPDLHSH HELKAIENCE YAFNLKKDEV CVNPYHYQRV ETPVLPPVLV PRHTEILTEL PPLDDYTHSI PENTNFPAGI EPQSNYIPET PPPGYISEDG ETSDQQLNQS MDTGSPAELS PTTLSPVNHS LDLQPVTYSE PAFWCSIAYY ELNQRVGETF HASQPSLTVD GFTDPSNSER FCLGLLSNVN RNATVEMTRR HIGRGVRLYY IGGEVFAECL SDSAIFVQSP NCNQRYGWHP ATVCKIPPGC NLKIFNNQEF AALLAQSVNQ GFEAVYQLTR MCTIRMSFVK GWGAEYRRQT VTSTPCWIEL HLNGPLQWLD KVLTQMGSPS VRCSSMS

Purity

>90%, by SDS-PAGE

Endotoxin Level

< 1.0 EU per 1 microgram of protein (determined by LAL method)

Predicted Molecular Mass

54.4 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Protein / Peptide Type

Recombinant Protein

Reviewed Applications

Read 1 review rated 5 using NBC1-25856 in the following applications:

Immunoassay Standard (1 Review)

Scientific Data Images for Recombinant Human Smad2 His Protein

SDS-PAGE: Recombinant Human Smad2 His Protein [NBC1-25856]

SDS-Page: Recombinant Human Smad2 Protein [NBC1-25856] - SMAD2, 54.4 kDa (487aa) with a purity of 95% by SDS - PAGE

Formulation, Preparation and Storage

NBC1-25856

Formulation	20 mM Tris-HCl buffer (pH8.0), 0.1 M NaCl, 20% glycerol
Preservative	No Preservative
Concentration	0.5 mg/ml
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Smad2

SMAD2(Mothers against decapentaplegic homolog 2) belongs to the SMAD family of proteins that mediate signal transduction by the TGF-beta/activin/BMP-2/4 cytokine superfamily from receptor Ser/Thr protein kinases at the cell surface to the nucleus. Phosphorylated Smad2 is then able to form a complex with Smad4 or SARA. These complexes accumulate in the cell nucleus, where they are directly participating in the regulation of gene expression. Recombinant SMAD2 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Long Name

Mothers Against DPP Homolog 2

Alternate Names

JV18-1, MADH2, MADR2, Smox

Gene Symbol

SMAD2

Additional Smad2 Products

Product Documents for Recombinant Human Smad2 His Protein

Product Datasheets

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COA

Product Specific Notices for Recombinant Human Smad2 His Protein

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

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