Recombinant Mouse Sonic Hedgehog/Shh Protein

Name: Recombinant Mouse Sonic Hedgehog/Shh Protein
Brand: Novus Biologicals
SKU: NBP2-35174

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-35174

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Product Specifications
Scientific Data
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Key Product Details

Source

E. coli

Accession #

Q62226

Conjugate

Unconjugated

Applications

Bioactivity, SDS-PAGE

View all Sonic Hedgehog/Shh Proteins and Enzymes »

Product Specifications

Description

A single non-glycosylated polypeptide chain containing 176 amino acids corresponding to Sonic Hedgehog/Shh Source: E. coli

Uniprot ID: Q62226

Amino Acid Sequence: IVIGPGRGFG KRRHPKKLTP LAYKQFIPNV AEKTLGASGR YEGKITRNSE RFKELTPNYN PDIIFKDEEN TGADRLMTQR CKDKLNALAI SVMNQWPGVK LRVTEGWDED GHHSEESLHY EGRAVDITTS DRDRSKYGML ARLAVEAGFD WVYYESKAHI HCSVKAENSV AAKSGG

This lyophilized preparation is stable at 2-8 degrees C, but should be kept at -20 degrees C for long term storage, preferably desiccated. Upon reconstitution, the preparation is most stable at -20 to -80 degrees C, and can be stored for one week at 2-8 degrees C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 degrees C to -80 degrees C. Avoid repeated freeze/thaw cycles.

Purity

>95%, by SDS-PAGE and HPLC

Endotoxin Level

Less than 1 EU/ug of Sonic Hedgehog/Shh as determined by LAL method.

Predicted Molecular Mass

19.8 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Activity

Sonic Hedgehog Protein is fully biologically active when compared to standard. The ED50 as determined by inducing alkaline phosphatase production of murine C3H10T1/2 cells is 0.5 - 1.0 ug/ml.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Mouse Sonic Hedgehog/Shh Protein

SDS-PAGE: Recombinant Mouse Sonic Hedgehog/Shh Protein [NBP2-35174]

SDS-Page: Mouse Sonic Hedgehog/Shh Protein [NBP2-35174]

Formulation, Preparation and Storage

NBP2-35174

Formulation	Lyophilized from a 0.2 um filtered concentrated solution in PBS, pH 7.4.
Preservative	No Preservative
Concentration	Lyoph
Reconstitution	Recommended to centrifuge prior to opening. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/mL. Apportion stock solutions into working aliquots and store at
Format	Carrier-Free
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles.

Background: Sonic Hedgehog/Shh

Sonic Hedgehog (Shh) is a secreted protein that regulates developmental morphogenesis of the central nervous system, somite, limb, whisker and hair follicles, foregut, tooth, and bone. Shh is also involved in regulating neural and hematopoeitic stem cell fates and determination of the T cell repertoire. Aberrant Shh signaling is implicated in the development of several malignancies. Shh undergoes autocatalytic processing that yields a palmitoylated N-terminal fragment and a C-terminal fragment. The N-terminal domain retains all known signaling capabilities, while the C-terminal domain acts as a cholesterol transferase. Shh activity is mediated by a multicomponent receptor complex that includes Patched and Smoothened. The N-terminal fragment of Shh is greater than 98% conserved between mouse, human, rat, canine, porcine, and chicken.

Alternate Names

HHG1, HLP3, HPE3, MCOPCB5, Shh, ShhNC, SMMCI, TPTPS

Gene Symbol

SHH

UniProt