Recombinant Human Sonic Hedgehog/Shh Protein

Name: Recombinant Human Sonic Hedgehog/Shh Protein
Brand: Novus Biologicals
SKU: NBP2-35265

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-35265

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Product Specifications
Scientific Data
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Background
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Key Product Details

Source

E. coli

Accession #

Q15465

Conjugate

Unconjugated

Applications

Bioactivity, SDS-PAGE

View all Sonic Hedgehog/Shh Proteins and Enzymes »

Product Specifications

Description

A single non-glycosylated polypeptide chain containing 176 amino acids corresponding to Sonic Hedgehog/Shh Source: E. coli

Uniprot ID: Q15465

Amino Acid Sequence: IVIGPGRGFG KRRHPKKLTP LAYKQFIPNV AEKTLGASGR YEGKISRNSE RFKELTPNYN PDIIFKDEEN TGADRLMTQR CKDKLNALAI SVMNQWPGVK LRVTEGWDED GHHSEESLHY EGRAVDITTS DRDRSKYGML ARLAVEAGFD WVYYESKAHI HCSVKAENSV AAKSGG

Purity

> 98 % pure by SDS-PAGE and HPLC

Endotoxin Level

Less than 1 EU/ug of Sonic Hedgehog/Shh as determined by LAL method.

Predicted Molecular Mass

19.8 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Activity

Sonic Hedgehog/Shh protein is fully biologically active when compared to standard. The ED50 as determined by inducing alkaline phosphatase production of murine C3H/10T1/2 cells is less than 1 ug/ml, corresponding to a specific activity of > 1.0 x 10^3 IU/mg

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Human Sonic Hedgehog/Shh Protein

SDS-PAGE: Recombinant Human Sonic Hedgehog/Shh Protein [NBP2-35265]

SDS-Page: Human Sonic Hedgehog/Shh Protein [NBP2-35265]

Formulation, Preparation and Storage

NBP2-35265

Formulation	Lyophilized from a 0.2 um filtered concentrated solution in 20 mM PB, pH 7.4, 150 mM NaCl.
Preservative	No Preservative
Concentration	Lyoph
Reconstitution	Recommended to centrifuge prior to opening. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/mL.
Format	Carrier-Free
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles.

Background: Sonic Hedgehog/Shh

Sonic Hedgehog (Shh) is a secreted protein that regulates developmental morphogenesis of the central nervous system, somite, limb, whisker and hair follicles, foregut, tooth, and bone. Shh is also involved in regulating neural and hematopoeitic stem cell fates and determination of the T cell repertoire. Aberrant Shh signaling is implicated in the development of several malignancies. Shh undergoes autocatalytic processing that yields a palmitoylated N-terminal fragment and a C-terminal fragment. The N-terminal domain retains all known signaling capabilities, while the C-terminal domain acts as a cholesterol transferase. Shh activity is mediated by a multicomponent receptor complex that includes Patched and Smoothened. The N-terminal fragment of Shh is greater than 98% conserved between mouse, human, rat, canine, porcine, and chicken.

Alternate Names

HHG1, HLP3, HPE3, MCOPCB5, Shh, ShhNC, SMMCI, TPTPS

Gene Symbol

SHH

UniProt