Goat IgG (H+L) Fluorescein-conjugated Antibody
R&D Systems, part of Bio-Techne | Catalog # F0109
Conjugate
Catalog #
Key Product Details
Species Reactivity
Goat
Applications
Flow Cytometry
Label
Fluorescein (Excitation = 488 nm, Emission = 515-545 nm)
Antibody Source
Polyclonal Donkey IgG
Product Specifications
Immunogen
Goat IgG
Specificity
Goat IgG specific IgG was purified by goat IgG (H+L) affinity chromatography.
Clonality
Polyclonal
Host
Donkey
Isotype
IgG
Scientific Data Images for Goat IgG (H+L) Fluorescein-conjugated Antibody
Detection of Goat IgG Primary Antibody by Flow Cytometry
Human peripheral blood lymphocytes were stained with Goat Anti-Human Common beta Chain Antigen Affinity-purified Polyclonal Antibody (Catalog # AF906, filled histogram) or control antibody (Catalog # AB-108-C, open histogram), followed by Fluorescein-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0109) at 10 uL/106cells.Applications for Goat IgG (H+L) Fluorescein-conjugated Antibody
Application
Recommended Usage
Flow Cytometry
10 µL/106 cells
Sample: Designed for use as a secondary developing reagent in immunofluorescent assays, such as flow cytometry, where the primary antibody does not have a fluorescent reporter, is of goat origin, and is of IgG class. The recommended concentration is 10 μL/106 cells.
Sample: Designed for use as a secondary developing reagent in immunofluorescent assays, such as flow cytometry, where the primary antibody does not have a fluorescent reporter, is of goat origin, and is of IgG class. The recommended concentration is 10 μL/106 cells.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8° C. Do not use past expiration date. Protect from light.
Background: IgG
R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels.
Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching. We are currently offering secondary antibodies recognizing mouse, rat, goat, sheep, and rabbit IgG as well as chicken IgY. These reagents are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy.
Long Name
Immunoglobulin G
Alternate Names
Immunoglobulin G, ImmunoglobulinG
Additional IgG Products
Product Documents for Goat IgG (H+L) Fluorescein-conjugated Antibody
Product Specific Notices for Goat IgG (H+L) Fluorescein-conjugated Antibody
For research use only
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