Goat anti-Human IgG Fc Secondary Antibody [Alkaline Phosphatase] (Pre-adsorbed)
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-74996
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human
Applications
ELISA, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Alkaline Phosphatase
Antibody Source
Polyclonal Goat IgG
Format
Pre-adsorbed
Concentration
1.5 mg/ml
Product Specifications
Immunogen
Purified Human IgG, Fc fragment
Reactivity Notes
Based on IEP, no reactivity is observed to non-immunoglobulin human serum immunoglobulins, light chains on all human immunoglobulins, and human IgA or IgM
Specificity
Based on IEP, this antibody reacts with heavy gamma chains on human IgG. This antibody has been pre-adsorbed against human IgA + IgM.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Description
Purity > 95% based on SDS-PAGE. Goat serum was obtained from healthy animals of US origin and under the care of a registered veterinarian.
Store undiluted liquid at 2-8C. For storage at -20C, dilute with an equal volume of glycerol to prevent loss of enzymatic activity. Prepare working dilutions prior to use and then discard.
Store undiluted liquid at 2-8C. For storage at -20C, dilute with an equal volume of glycerol to prevent loss of enzymatic activity. Prepare working dilutions prior to use and then discard.
Applications
Application
Recommended Usage
ELISA
1:200-1:5000
Immunohistochemistry
1:500-1:5000
Western Blot
1:200-1:5000
Application Notes
This conjugate is suitable for all immunoassay applications.
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
30 mM Triethanolamine, pH 7.2, 5 mM Magnesium Chloride, 0.1 mM Zinc Chloride, 1 % (w/v) BSA, Protease/IgG free
Format
Pre-adsorbed
Preservative
0.05% Sodium Azide
Concentration
1.5 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C. After dilution, add an equal volume of glycerol to prevent loss of enzymatic activity and store at -20C. Avoid repeated freeze-thaw cycles.
Background: IgG Fc
UniProt
Additional IgG Fc Products
Product Specific Notices
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.
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