Goat anti-Human IgG Fc Secondary Antibody [Biotin] (Pre-adsorbed)
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-74997
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human
Applications
ELISA, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Biotin
Antibody Source
Polyclonal Goat IgG
Format
Pre-adsorbed
Concentration
1.5 mg/ml
Product Summary for Goat anti-Human IgG Fc Secondary Antibody [Biotin] (Pre-adsorbed)
Immunogen
Purified Human IgG, Fc fragment
Reactivity Notes
Based on IEP, no reactivity is observed to non-immunoglobulin human serum immunoglobulins, light chains on all human immunoglobulins, and human IgA or IgM
Specificity
Based on IEP, this antibody reacts with heavy gamma chains on human IgG. This antibody has been pre-adsorbed against human IgA + IgM.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Description
Purity > 95% based on SDS-PAGE. Goat serum was obtained from healthy animals of US origin and under the care of a registered veterinarian.
Applications for Goat anti-Human IgG Fc Secondary Antibody [Biotin] (Pre-adsorbed)
Application
Recommended Usage
ELISA
1:2000 - 1:20000
Immunohistochemistry
1:500 - 1:5000
Western Blot
1:2000 - 1:20000
Application Notes
This conjugate is suitable for all immunoassay applications.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free
Format
Pre-adsorbed
Preservative
0.05% Sodium Azide
Concentration
1.5 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C. Do not freeze.
Background: IgG Fc
UniProt
Additional IgG Fc Products
Product Documents for Goat anti-Human IgG Fc Secondary Antibody [Biotin] (Pre-adsorbed)
Product Specific Notices for Goat anti-Human IgG Fc Secondary Antibody [Biotin] (Pre-adsorbed)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.
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