Rabbit anti-Mouse IgG1 Heavy Chain Secondary Antibody [Rhodamine]
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-73629
Conjugate
Catalog #
Key Product Details
Species Reactivity
Mouse
Applications
Dot Blot, Flow Cytometry, Fluorophore-linked immunosorbent assay, Immunocytochemistry/ Immunofluorescence
Label
Rhodamine (Excitation = 570 nm, Emission = 590 nm)
Antibody Source
Polyclonal Rabbit IgG
Concentration
LYOPH mg/ml
Product Summary for Rabbit anti-Mouse IgG1 Heavy Chain Secondary Antibody [Rhodamine]
Immunogen
Mouse IgG1 heavy chain
Specificity
Specificity was confirmed by ELISA at less than 1% cross reactivity against other mouse heavy or light chain isotypes.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Description
Store vial at 4C prior to restoration. For extended storage aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.
This product was prepared from monospecific antiserum by immunoaffinity chromatography using antigens coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum and Mouse IgG and Mouse Serum
This product was prepared from monospecific antiserum by immunoaffinity chromatography using antigens coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum and Mouse IgG and Mouse Serum
Scientific Data Images for Rabbit anti-Mouse IgG1 Heavy Chain Secondary Antibody [Rhodamine]
Dot Blot: Rabbit anti-Mouse IgG1 Heavy Chain Secondary Antibody [Rhodamine] [NBP1-73629] - Antigen: Mouse IgG1. Load: Lane 1 - 50 ng Lane 2 - 16.67 ng Lane 3 - 5.56 ng Lane 4 - 1.85 ng Lane 5 - 0.62 ng. Incubated for 1hr at RT; blocked for 60 min at RT.
Applications for Rabbit anti-Mouse IgG1 Heavy Chain Secondary Antibody [Rhodamine]
Application
Recommended Usage
Flow Cytometry
1:500 - 1:2500
Fluorophore-linked immunosorbent assay
1:10000 - 1:50000
Immunocytochemistry/ Immunofluorescence
1:1000 - 1:5000
Application Notes
This product has been tested by dot blot and is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Multi-step
Reconstitution
Reconstitute with 1.0 ml deionized water (or equivalent).
Formulation
Lyophilized from 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative
0.01% Sodium Azide
Concentration
LYOPH mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store lyophilized antibody at 4C in the dark. Aliquot reconstituted liquid and store at -20C. Avoid freeze-thaw cycles.
Background: IgG1 Heavy Chain
Additional IgG1 Heavy Chain Products
Product Documents for Rabbit anti-Mouse IgG1 Heavy Chain Secondary Antibody [Rhodamine]
Product Specific Notices for Rabbit anti-Mouse IgG1 Heavy Chain Secondary Antibody [Rhodamine]
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.
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