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Key Product Details

Species Reactivity

Mouse

Applications

ELISA, Immunohistochemistry, Western Blot

Label

HRP

Antibody Source

Polyclonal Rabbit IgG

Concentration

LYOPH mg/ml

Product Summary for Rabbit anti-Mouse IgG1 Heavy Chain Secondary Antibody [HRP]

Immunogen

Mouse IgG1 heavy chain

Specificity

Specificity was confirmed by ELISA against other mouse or human heavy chain isotypes. Cross reactivity with Mouse IgG2b and IgG3 is 1%.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

Store vial at 4C prior to restoration. For extended storage aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.

This product was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum, Mouse IgG and Mouse Serum

Scientific Data Images for Rabbit anti-Mouse IgG1 Heavy Chain Secondary Antibody [HRP]

Western Blot: Rabbit anti-Mouse IgG1 Heavy Chain Secondary Antibody [HRP] [NBP1-73741] - Lane 1: Mouse IgG1. Lane 2: None. Load: 50 ng per lane. Primary antibody: None. Secondary antibody: Peroxidase rabbit secondary antibody at 1:1,000 for 60 min at RT. Blocking: incubated with blocking buffer for 30 min at RT. Predicted/Observed size: 55 kDa, 55 kDa for Mouse IgG1 (Gamma 1 chain). Other band(s): None.
Rabbit anti-Mouse IgG1 Heavy Chain Secondary Antibody [HRP]

Rabbit anti-Mouse IgG1 Heavy Chain Secondary Antibody [HRP]

Western blot of Peroxidase conjugated Rabbit Anti-Mouse IgG1 (Gamma 1 chain) secondary antibody. Lane 1: Mouse IgG1. Lane 2: None. Load: 50 ng per lane. Primary antibody: None. Secondary antibody: Peroxidase rabbit secondary antibody at 1:1,000 for 60 min at RT. Blocking: for 30 min at RT. Predicted/Observed size: 55 kDa, 55 kDa for Mouse IgG1 (Gamma 1 chain). Other band(s): None.

Applications for Rabbit anti-Mouse IgG1 Heavy Chain Secondary Antibody [HRP]

Application
Recommended Usage

ELISA

1:20000 - 1:200000

Immunohistochemistry

1:500 - 1:2500

Western Blot

1:2000 - 1:20000
Application Notes
This product is available in a variety of formats. Anti-Mouse IgG1 Peroxidase Antibody has been tested by ELISA and western blot and is suitable for ELISA, Immunohistochemistry western blotting as well as other anti IgG1 antibody based assays.

NOTE: Do NOT add Sodium Azide (it inhibits HRP irreversibly).
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Multi-step

Reconstitution

Reconstitute with 1.0 ml deionized water (or equivalent).

Formulation

Lyophilized from 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative

0.01% Gentamicin Sulfate

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store lyophilized antibody at 4C in the dark. Aliquot reconstituted liquid and store at -20C. Avoid freeze-thaw cycles.

Background: IgG1 Heavy Chain

Additional IgG1 Heavy Chain Products

Product Documents for Rabbit anti-Mouse IgG1 Heavy Chain Secondary Antibody [HRP]

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Rabbit anti-Mouse IgG1 Heavy Chain Secondary Antibody [HRP]

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.

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