Key Product Details

Species Reactivity

Mouse

Applications

Fluorophore-linked immunosorbent assay, Immunocytochemistry/ Immunofluorescence, Western Blot

Label

DyLight 800 (Excitation = 777 nm, Emission = 794 nm)

Antibody Source

Polyclonal Rabbit IgG

Concentration

LYOPH mg/ml

View all IgG2a Heavy Chain Secondary Antibodies »

Product Summary for Rabbit anti-Mouse IgG2a Heavy Chain Secondary Antibody [DyLight 800]

Immunogen

Mouse IgG2a heavy chain

Specificity

Minimal cross reactivity was noted against other Mouse immunoglobulin classes or subclasses.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

Store vial at 4C prior to restoration. For extended storage aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.

This product was prepared from monospecific antiserum by immunoaffinity chromatography using antigens coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum, Mouse IgG and Mouse Serum

Scientific Data Images for Rabbit anti-Mouse IgG2a Heavy Chain Secondary Antibody [DyLight 800]

Fluorophore-linked immunosorbent assay: Rabbit anti-Mouse IgG2a Heavy Chain Secondary Antibody [DyLight 800] [NBP1-72915] - Fluorescence absorption and emission spectra of Dylight 800 in PBS, pH 7.2.
Rabbit anti-Mouse IgG2a Heavy Chain Secondary Antibody [DyLight 800]

Rabbit anti-Mouse IgG2a Heavy Chain Secondary Antibody [DyLight 800]

DyLight(TM) dyes can be used for two-color Western Blot detection with low background and high signal. Anti-tubulin was detected using a DyLight(TM) 680 conjugate. Anti-TNFa was detected using a DyLight(TM) 800 conjugate. The image was captured using the Odyssey(R) Infrared Imaging System developed by LI-COR.

Applications for Rabbit anti-Mouse IgG2a Heavy Chain Secondary Antibody [DyLight 800]

Application
Recommended Usage

Fluorophore-linked immunosorbent assay

1:20000

Immunocytochemistry/ Immunofluorescence

1:5000

Western Blot

1:10000
Application Notes
This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms. The emission spectra for this DyLight(TM) conjugate match the principle output wavelengths of most common fluorescence instrumentation.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Multi-step

Reconstitution

Reconstitute with 100 ul deionized water (or equivalent).

Formulation

Lyophilized from 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative

0.01% Sodium Azide

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store lyophilized antibody at 4C in the dark. Aliquot reconstituted liquid and store at -20C. Avoid freeze-thaw cycles.

Product Documents for Rabbit anti-Mouse IgG2a Heavy Chain Secondary Antibody [DyLight 800]

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Rabbit anti-Mouse IgG2a Heavy Chain Secondary Antibody [DyLight 800]

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.

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