Rabbit anti-Mouse IgG2a Heavy Chain Secondary Antibody [DyLight 405]
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-72921
Conjugate
Catalog #
Key Product Details
Species Reactivity
Mouse
Applications
Fluorophore-linked immunosorbent assay, Immunocytochemistry/ Immunofluorescence, Western Blot
Label
DyLight 405 (Excitation = 400 nm, Emission = 420 nm)
Antibody Source
Polyclonal Rabbit IgG
Concentration
LYOPH mg/ml
Product Summary for Rabbit anti-Mouse IgG2a Heavy Chain Secondary Antibody [DyLight 405]
Immunogen
Mouse IgG2a heavy chain
Specificity
Minimal cross reactivity was noted against other Mouse immunoglobulin classes or subclasses.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Description
Store vial at 4C prior to restoration. For extended storage aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.
This product was prepared from monospecific antiserum by immunoaffinity chromatography using antigens coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum, Mouse IgG and Mouse Serum
This product was prepared from monospecific antiserum by immunoaffinity chromatography using antigens coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum, Mouse IgG and Mouse Serum
Scientific Data Images for Rabbit anti-Mouse IgG2a Heavy Chain Secondary Antibody [DyLight 405]
Fluorophore-linked immunosorbent assay: Rabbit anti-Mouse IgG2a Heavy Chain Secondary Antibody [DyLight 405] [NBP1-72921] - Rabbit anti-Mouse IgG2a Heavy Chain Secondary antibody [DyLight 405]
Applications for Rabbit anti-Mouse IgG2a Heavy Chain Secondary Antibody [DyLight 405]
Application
Recommended Usage
Fluorophore-linked immunosorbent assay
1:20000
Immunocytochemistry/ Immunofluorescence
1:5000
Western Blot
1:10000
Application Notes
This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms. The emission spectra for this DyLight(TM) conjugate match the principle output wavelengths of most common fluorescence instrumentation.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Multi-step
Reconstitution
Reconstitute with 100 ul deionized water (or equivalent).
Formulation
Lyophilized from 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative
0.01% Sodium Azide
Concentration
LYOPH mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store lyophilized antibody at 4C in the dark. Aliquot reconstituted liquid and store at -20C. Avoid freeze-thaw cycles.
Background: IgG2a Heavy Chain
Additional IgG2a Heavy Chain Products
Product Documents for Rabbit anti-Mouse IgG2a Heavy Chain Secondary Antibody [DyLight 405]
Product Specific Notices for Rabbit anti-Mouse IgG2a Heavy Chain Secondary Antibody [DyLight 405]
DyLight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.
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