Rabbit anti-Mouse IgG3 Heavy Chain Secondary Antibody [Alkaline Phosphatase]
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-72675
Conjugate
Catalog #
Key Product Details
Species Reactivity
Mouse
Applications
ELISA, Immunohistochemistry, Western Blot
Label
Alkaline Phosphatase
Antibody Source
Polyclonal Rabbit IgG
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Mouse IgG3 heavy chain
Specificity
Specificity was confirmed by ELISA at less than 1% cross reactivity against other mouse heavy or light chain isotypes.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Description
Store vial at 4C before opening. DO NOT FREEZE. This product is stable at 4C as an undiluted liquid. Dilute only prior to immediate use. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.
This product was prepared from monospecific antiserum by immunoaffinity chromatography using antigens coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase (calf intestine) and anti-Rabbit Serum
This product was prepared from monospecific antiserum by immunoaffinity chromatography using antigens coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase (calf intestine) and anti-Rabbit Serum
Applications
Application
Recommended Usage
ELISA
1:10000 - 1:30000
Immunohistochemistry
1:200 - 1:1000
Western Blot
1:500 - 1:2500
Application Notes
This product has been tested by ELISA and dot blot and is assayed against 1.0 ug of Mouse IgG in a standard capture ELISA using pNPP p-nitrophenyl phosphate as a substrate for 30 minutes at room temperature. A working dilution of 1:2,000 to 1:4,000 is suggested for this product.
Formulation, Preparation, and Storage
Purification
Multi-step
Formulation
0.05 M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50% (v/v) Glycerol; pH 8.0, 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative
0.01% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C. Do not freeze.
Background: IgG3 Heavy Chain
Additional IgG3 Heavy Chain Products
Product Specific Notices
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.
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