Rabbit anti-Mouse IgG3 Heavy Chain Secondary Antibody [Biotin]
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-73701
Conjugate
Catalog #
Key Product Details
Species Reactivity
Mouse
Applications
ELISA, Immunohistochemistry, Western Blot
Label
Biotin
Antibody Source
Polyclonal Rabbit IgG
Concentration
LYOPH mg/ml
Product Specifications
Immunogen
Mouse IgG3 heavy chain
Specificity
Specificity was confirmed by ELISA. Typically less than 1% cross reactivity was observed against other Mouse heavy chain isotypes.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Description
Store vial at 4C prior to restoration. For extended storage aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse antigens coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-biotin, anti-Rabbit Serum, Mouse IgG and Mouse Serum
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse antigens coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-biotin, anti-Rabbit Serum, Mouse IgG and Mouse Serum
Applications for Rabbit anti-Mouse IgG3 Heavy Chain Secondary Antibody [Biotin]
Application
Recommended Usage
ELISA
1:8000 - 1:32000
Immunohistochemistry
1:500 - 1:2000
Western Blot
1:1000 - 1:5000
Application Notes
This product has been tested by ELISA and is ideal for ELISA, western blotting, Immunohistochemistry, Fluorescence Microscopy, Flow Cytometry as well as other antibody detection methods.
Formulation, Preparation, and Storage
Purification
Multi-step
Reconstitution
Reconstitute with 1.0 ml deionized water (or equivalent).
Formulation
Lyophilized from 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative
0.01% Sodium Azide
Concentration
LYOPH mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store lyophilized antibody at 4C in the dark. Aliquot reconstituted liquid and store at -20C. Avoid freeze-thaw cycles.
Background: IgG3 Heavy Chain
Additional IgG3 Heavy Chain Products
Product Documents for Rabbit anti-Mouse IgG3 Heavy Chain Secondary Antibody [Biotin]
Product Specific Notices for Rabbit anti-Mouse IgG3 Heavy Chain Secondary Antibody [Biotin]
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.
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