Goat anti-Monkey IgM Heavy Chain Secondary Antibody [HRP]
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-73726
![](https://resources.bio-techne.com/images/products/Goat anti-Monkey IgM Heavy Chain Secondary Antibody [HRP]-Western Blot-NBP1-73726-img0004.jpg)
Conjugate
Catalog #
Key Product Details
Species Reactivity
Monkey
Applications
ELISA, Immunohistochemistry, Western Blot
Label
HRP
Antibody Source
Polyclonal Goat IgG
Concentration
LYOPH mg/ml
Product Specifications
Immunogen
Monkey IgM whole molecule
Specificity
No reaction was observed against other Monkey heavy or light chain proteins.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Description
Store vial at 4C prior to restoration. For extended storage aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Monkey IgM coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum, Monkey IgM and Monkey Serum
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Monkey IgM coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum, Monkey IgM and Monkey Serum
Scientific Data Images for Goat anti-Monkey IgM Heavy Chain Secondary Antibody [HRP]
Western Blot: Goat anti-Monkey IgM Heavy Chain Secondary Antibody [HRP] [NBP1-73726] - Western Blot of Goat anti-Monkey IgM Heavy Chain Secondary antibody [HRP]. Lane 1: Monkey IgM. Lane 2: none. Load: 100 ng per lane.Primary antibody: none.Secondary antibody: Peroxidase goat secondary antibody at 1:1,000 for 60 min at RT.Block for 30 min at RT.Observed size: 72 kDa for Rabbit IgG. Other band(s): none.
N/A: Goat anti-Monkey IgM Heavy Chain Secondary Antibody [HRP] [NBP1-73726]
Goat anti-Monkey IgM Heavy Chain Secondary Antibody [HRP]
Western Blot of Peroxidase conjugated Goat anti-Monkey IgM mu antibody. Lane 1: Monkey IgM. Lane 2: none. Load: 100 ng per lane. Primary antibody: none. Secondary antibody: Peroxidase goat secondary antibody at 1:1,000 for 60 min at RT. Block for 30 min at RT. Observed size: 72 kDa for Rabbit IgG. Other band(s): none.Applications for Goat anti-Monkey IgM Heavy Chain Secondary Antibody [HRP]
Application
Recommended Usage
ELISA
1:65000 - 1:160000
Immunohistochemistry
1:500 -1:2500
Western Blot
1:1000 - 1:5000
Application Notes
This product has been tested by ELISA, dot blot, and western blot and is suitable for immunoblotting (western or dot blot), ELISA, immunoperoxidase electron microscopy and immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency.
NOTE: Do NOT add Sodium Azide (it inhibits HRP irreversibly).
NOTE: Do NOT add Sodium Azide (it inhibits HRP irreversibly).
Formulation, Preparation, and Storage
Purification
Multi-step
Reconstitution
Reconstitute with 1.0 ml deionized water (or equivalent).
Formulation
Lyophilized from 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative
0.01% Gentamicin Sulfate
Concentration
LYOPH mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store lyophilized antibody at 4C in the dark. Aliquot reconstituted liquid and store at -20C. Avoid freeze-thaw cycles.
Background: IgM Heavy Chain
Alternate Names
Constant region of heavy chain of IgM, Ig Mu Chain C Region BOT, Ig Mu Chain C Region GAL, Ig Mu Chain C Region OU, immunoglobulin heavy constant mu, Immunoglobulin mu chain
Gene Symbol
IGHM
Additional IgM Heavy Chain Products
Product Documents for Goat anti-Monkey IgM Heavy Chain Secondary Antibody [HRP]
Product Specific Notices for Goat anti-Monkey IgM Heavy Chain Secondary Antibody [HRP]
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.
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