Key Product Details

Species Reactivity

Monkey

Applications

ELISA, Immunohistochemistry, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all IgM Heavy Chain Secondary Antibodies »

Product Specifications

Immunogen

Monkey IgM whole molecule

Specificity

No reaction was observed against other monkey heavy or light chain proteins.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Description

This product was prepared from monospecific antiserum by immunoaffinity chromatography using Monkey IgM coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Monkey IgM and Monkey Serum

Store vial at 4C prior to opening. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing.

Scientific Data Images for Goat anti-Monkey IgM Heavy Chain Secondary Antibody

Western Blot: Goat anti-Monkey IgM Heavy Chain Secondary Antibody [NBP2-59719]

Western Blot: Goat anti-Monkey IgM Heavy Chain Secondary Antibody [NBP2-59719]

Western Blot: Goat anti-Monkey IgM Heavy Chain Secondary Antibody [NBP2-59719] - Western Blot of Goat anti-Monkey IgM Heavy Chain Secondary antibody. Lane 1: Monkey IgM. Lane 2: Monkey IgG.Lane 3: Monkey IgG (A and M Free).Lane 4: Monkey IgG F(c). Load: 50 ng per lane.Primary antibody: Goat anti-Monkey IgM Heavy Chain Secondary antibody at 1:1,000 for 1 hour at RT.Secondary antibody: Peroxidase conjugated Dk-a-Gt IgG at 1:40,000 for 30 min at RT.Block for 30 min at RT.Predicted/Observed size: 70 kDa, 70 kDa for Monkey IgM (mu chain).
Western Blot: Goat anti-Monkey IgM Heavy Chain Secondary Antibody [NBP2-59719]

Western Blot: Goat anti-Monkey IgM Heavy Chain Secondary Antibody [NBP2-59719]

Western Blot: Goat anti-Monkey IgM Heavy Chain Secondary Antibody [NBP2-59719] - Lane 1: Monkey IgM. Lane 2: None. Load: 50 ng per lane. Primary antibody: None. Secondary antibody: Fluorescein goat secondary antibody at 1:1,000 for 60 min at RT. Block: incubated with blocking buffer for 30 min t RT. Predicted/Observed size: 72 kDa, 72 kDa for Monkey IgM. Other band(s): None. Image using the Fluorescein form of this antibody.
N/A: Goat anti-Monkey IgM Heavy Chain Secondary Antibody [NBP2-59719]

Applications for Goat anti-Monkey IgM Heavy Chain Secondary Antibody

Application
Recommended Usage

ELISA

1:15000 - 1:100000

Immunohistochemistry

1-500-1:2500

Western Blot

1:1000-1:5000
Application Notes
This product has been tested by ELISA, SDS-PAGE, dot blot, and western blot and is suitable for use in immunoelectrophoresis, western-blot, competitive western-blot, ELISA and competitive ELISA assays. Specific conditions for reactivity and signal detection should be optimized by the end user.

Formulation, Preparation, and Storage

Purification

Multi-step

Formulation

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Preservative

0.01% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: IgM Heavy Chain

Alternate Names

Constant region of heavy chain of IgM, Ig Mu Chain C Region BOT, Ig Mu Chain C Region GAL, Ig Mu Chain C Region OU, immunoglobulin heavy constant mu, Immunoglobulin mu chain

Gene Symbol

IGHM

Additional IgM Heavy Chain Products

Product Documents for Goat anti-Monkey IgM Heavy Chain Secondary Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for Goat anti-Monkey IgM Heavy Chain Secondary Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.

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