Mouse IgE Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB9935
Key Product Details
Species Reactivity
Mouse
Applications
Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Rat IgG2A Clone # 343527
Product Specifications
Immunogen
Mouse IgE
Specificity
Detects mouse IgE in Direct Elisa and Western blots.
Clonality
Monoclonal
Host
Rat
Isotype
IgG2A
Scientific Data Images for Mouse IgE Antibody
Detection of Mouse IgE by Western Blot.
Western blot shows lysates of purified Mouse IgM (negative control), purified Mouse IgA (negative control), purified Mouse IgG(negative control), and human purified Mouse IgE. PVDF membrane was probed with 2 µg/mL of Rat Anti-Mouse IgE Monoclonal Antibody (Catalog # MAB9935) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for IgE at approximately 75 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.Applications for Mouse IgE Antibody
Application
Recommended Usage
Western Blot
2 µg/mL
Sample: Purified mouse IgE
Sample: Purified mouse IgE
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: IgE
R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels.
Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching. We are currently offering secondary antibodies recognizing mouse, rat, goat, sheep, and rabbit IgG as well as chicken IgY. These reagents are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy.
Long Name
Immunoglobulin E
Alternate Names
IgE, immunoglobulin heavy constant epsilon
Additional IgE Products
Product Documents for Mouse IgE Antibody
Product Specific Notices for Mouse IgE Antibody
For research use only
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