Rat IgG NorthernLights™ NL637-conjugated Antibody

R&D Systems, part of Bio-Techne | Catalog # NL014

NorthernLights 637 Fluorochrome-labeled Antibody, X Absorbed
R&D Systems, part of Bio-Techne

Key Product Details

Species Reactivity

Rat

Applications

Flow Cytometry, Immunocytochemistry, Immunohistochemistry

Label

NL637 (Excitation = 637 nm, Emission = 658 nm)

Antibody Source

Polyclonal Goat IgG

View all IgG Secondary Antibodies »

Product Specifications

Immunogen

Rat IgG heavy and light chains

Specificity

Rat IgG (H+L)

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Rat IgG NorthernLights™ NL637-conjugated Antibody

Detection of Rat IgG Primary Antibody by Immunocytochemistry.

Detection of human Vimentin by incubating cells with 8 μg/mL of Rat anti-Human Vimentin monoclonal antibody (MAB2105) at room temperature for 1 hour followed by incubation with 1:200 dilution of Donkey Anti-Rat IgG NorthernLights™ NL637-conjugated secondary antibody for 1 hour at room temperature. Note strong positive staining in A172 cells (left panel, positive control) and a lack of non-specific background staining in the absence of primary antibodies (right panel, negative control).

Applications for Rat IgG NorthernLights™ NL637-conjugated Antibody

Application
Recommended Usage

Flow Cytometry

Use at a 1:200 dilution

Immunocytochemistry

Use at a 1:200 dilution

Immunohistochemistry

Use at a 1:200 dilution

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

*Contains <0.1% Sodium Azide, which is not hazardous at this concentration according to GHS classifications. Refer to the Safety Data Sheet (SDS) for additional information and handling instructions.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8° C. Do not use past expiration date. Protect from light.

Background: IgG

R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels.Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching. We are currently offering secondary antibodies recognizing mouse, rat, goat, sheep, and rabbit IgG as well as chicken IgY. These reagents are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy.

Long Name

Immunoglobulin G

Alternate Names

Immunoglobulin G, ImmunoglobulinG

Additional IgG Products

Product Documents for Rat IgG NorthernLights™ NL637-conjugated Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Rat IgG NorthernLights™ NL637-conjugated Antibody

For research use only

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