CoraFluor™ 2, Haloalkane
Tocris Bioscience, part of Bio-Techne | Catalog # 8818
Key Product Details
| Description: | Cell permeable terbium cryptate FRET donor for TR-FRET assay development, with chloroalkane ligand |
| Molecular Weight: | 1662.7 |
Biological Activity for CoraFluor™ 2, Haloalkane
CoraFluor™ 2, Haloalkane is a cell permeable terbium-based time-resolved Förster resonance energy transfer (TR-FRET) donor for live cell TR-FRET assay development, such as target engagement and ternary complex assays. CoraFluor™ 2, Haloalkane is designed with a chloroalkane ligand for reactivity with haloalkane dehalogenase labeled proteins. CoraFluor™ 2, Haloalkane can be used in live cell intracellular TR-FRET assays. It is exceptionally bright, providing unmatched assay sensitivity and stability in biological media over other commercially available TR-FRET donors. CoraFluor™ 2, Haloalkane displays red shifted excitation and is excitable by both 365 nm and 405 nm lasers. It is compatible with common acceptor dyes such as FAM/FITC, AF488, GFP, mCherry, TMR and Cyanine 5, SE (Cat. No. 5436), enabling seamless assay integration in high throughput screening applications.
CoraFluor™ 1, Haloalkane, CoraFluor™ 1, thiol reactive, CoraFluor™ 1, amine reactive and CoraFluor™ 2, amine reactive also available.
CoraFluor is a trademark of Bio-Techne Corp.
Licensing Information
Sold for research purposes only under agreement from Massachusetts General Hospital. US patent 2022/0025254
Optical Data for CoraFluor™ 2, Haloalkane
| λabs | 356 nm |
|---|---|
| Extinction Coefficient (ε) | 15900 M-1cm-1 |
| Quantum Yield (φ) | 0.35 |
| Closest Laser line | 337 nm |
| Cell Permeable | Yes |
| Application | Time-resolved fluorescence energy transfer (TR-FRET) |
|
Live cell TR-FRET assay. Cellular dose-dependent inhibition of HDAC1-dehalogenase fusion protein labeled with CoraFluor™ 2, Haloalkane in HEK293T cells by various HDAC inhibitors (treatment for 4 h at 37 °C, 1 μM SAHA-NCT). |