FAI

Name: FAI
Brand: Tocris Bioscience
SKU: 8011

Tocris Bioscience, part of Bio-Techne | Catalog # 8011

COA / SDS

Reagent for RNA SHAPE-MaP experiments in live cells

Key Product Details

Description:	Reagent for RNA SHAPE-MaP experiments in live cells
Chemical Name:	1H-Imidazol-1-yl(2-methyl-3-furanyl)methanone
Molecular Weight:	176.17

View Full Technical Details for FAI

Technical Data
Citations

Biological Activity for FAI

FAI is a reagent for RNA SHAPE-MaP experiments in live cells. Can be used to map RNA structures in vivo by forming stable 2'-OH adducts with RNA, blocking reverse transcriptase elongation. In vitro, FAI activity can be quenched with Dithiothreitol (Cat. No. 3154) and can be used to probe the secondary structure of mouse embryonic stem cell 5S rRNA. At elevated temperatures, FAI forms more stable adducts and exhibits less hydrolysis than NAI (Cat. No 7004).

Technical Data for FAI

M. Wt	176.17
Formula	C₉H₈N₂O₂
Storage	Store at -20°C
CAS Number	1415238-77-5
PubChem ID	66563653
InChi Key	OOBPIWAAJBRELM-UHFFFAOYSA-N
Smiles	O=C(C=1C=COC1C)N2C=NC=C2

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility for FAI

	Solvent	Max Conc. mg/mL	Max Conc. mM
Solubility
Solubility	DMSO	17.62	100

Preparing Stock Solutions for FAI

The following data is based on the product molecular weight 176.17.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
1 mM	5.68 mL	28.38 mL	56.76 mL
5 mM	1.14 mL	5.68 mL	11.35 mL
10 mM	0.57 mL	2.84 mL	5.68 mL
50 mM	0.11 mL	0.57 mL	1.14 mL

Molarity Calculator

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Reconstitution Calculator

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References for FAI

References are publications that support the biological activity of the product.

Spitale RNA SHAPE analysis in living cells. Nat.Chem.Biol. 2013 PMID: 23178934
Chan Comparative analysis reveals furoyl in vivo selective hydroxyl acylation analyzed by primer extension reagents form stable ribosyl ester adducts. Biochemistry 2017 PMID: 28319368

FAI