Pyridone 6

Name: Pyridone 6
Brand: Tocris Bioscience
SKU: 6577

Tocris Bioscience, part of Bio-Techne | Catalog # 6577

Citations (10)

COA / SDS

Potent pan-JAK inhibitor; induces intermediate mesoderm; cell-permeable

Key Product Details

Description:	Potent pan-JAK inhibitor; induces intermediate mesoderm; cell-permeable
Alternative Names:	JAK inhibitor I
Chemical Name:	2-(1,1-Dimethylethyl)-9-fluoro-1,6-dihydro-7H-benz[h]imidazo[4,5-f]isoquinolin-7-one
Purity:	≥98% (HPLC)
Molecular Weight:	309.34

View Full Technical Details for Pyridone 6

Technical Data
Citations

Biological Activity for Pyridone 6

Pyridone 6 is a potent pan-JAK inhibitor; ATP-competitive inhibitor of JAK 1/2/3 and Tyk2 (IC₅₀ values are 1, 5, 15 and 1 nM, respectively). Inhibits other kinases tested at 130 nM to >10 μM. Inhibits IL-2- and IL-4-dependent proliferation of CTLL cells and blocks STAT5 phosphorylation. Also inhibits Th1 and Th2 development, and promotes Th17 differentiation from naive T cells. When used in combination with Retinoic acid, LY 294002 and CCG 1423, it induces intermediate mesoderm differentiation from ESCs. Inhibits growth of primary myeloma cells grown in the presence of bone marrow stromal cells. Cell-permeable.

Compound Libraries for Pyridone 6

Pyridone 6 is also offered as part of the Tocriscreen 2.0 Max, Tocriscreen Kinase Inhibitor Library, Tocriscreen Antiviral Library, Tocriscreen Epigenetics Library and Tocriscreen Stem Cell Library. Find out more about compound libraries available from Tocris.

Technical Data for Pyridone 6

M. Wt	309.34
Formula	C₁₈H₁₆FN₃O
Storage	Store at -20°C
Purity	≥98% (HPLC)
CAS Number	457081-03-7
PubChem ID	5494425
InChi Key	VNDWQCSOSCCWIP-UHFFFAOYSA-N
Smiles	CC(C)(C1=NC2=C3C=CNC(C3=C4C=C(C=CC4=C2N1)F)=O)C

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility for Pyridone 6

	Solvent	Max Conc. mg/mL	Max Conc. mM
Solubility
	DMSO	30.93	100
	ethanol	15.47	50

Preparing Stock Solutions for Pyridone 6

The following data is based on the product molecular weight 309.34.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
1 mM	3.23 mL	16.16 mL	32.33 mL
5 mM	0.65 mL	3.23 mL	6.47 mL
10 mM	0.32 mL	1.62 mL	3.23 mL
50 mM	0.06 mL	0.32 mL	0.65 mL

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References for Pyridone 6

References are publications that support the biological activity of the product.

Thompson Photochemical preparation of a pyridone containing tetracycle: a Jak protein kinase inhibitor. Bioorg.Med.Chem.Lett. 2002 PMID: 11934592
Mae Combination of small molecules enhances differentiation of mouse embryonic stem cells into intermediate mesoderm through BMP7-positive cells. Biochem.Biophys.Res.Commun. 2010 PMID: 20171952