Simple Plex Expedites Process-Related Impurity Testing and Viral Titer Quantification
Fast and reproducible in-process data collection is essential during therapeutic manufacturing workflows. See how you can increase your product development bandwidth today. Automated Simple Plex™ assays on the Ella Platform:
- Reduce manual steps by 80%
- Enable data collection in 90 minutes or less
- Have picogram sensitivity
Host Cell Protein Impurity Testing
Host Cell Protein Impurity Testing
CHO Host Cell Protein Impurity Testing
Chinese Hamster Ovary (CHO) cells are commonly used during the production of therapeutic proteins or biologics. Host cell proteins (HCPs) are generated by CHO cells during this process. They are a major process-related impurity and need to be monitored and reduced at every step of the purification process. Simple Plex assays automate the CHO HCP in-process testing workflow and provide high-quality consistent results.
Viral Titer Quantification
Viral Titer Quantification
Adeno-Associated Virus Titration
Adeno-associated virus (AAV) is a popular viral vector in gene transduction workflows because it readily infects many cell types. Here, we pair Simple Plex™ viral titer assays on Ella™ with world-class Progen antibodies. The Simple Plex AAV viral titer immunoassay offers the consistency, precision, and dynamic range that you have come to expect, in less than 90 minutes.
HEK 293 Host Cell Protein Impurity Testing
Human Embryonic Kidney (HEK) 293 cells are often used as host cell lines for protein production or viral vector scale-up, as part of bio-therapeutic or cell and gene therapy manufacturing workflows. HEK 293 host cell proteins (HCPs) need to be monitored and reduced at every step of the manufacturing workflow. Residual HCPs that go undetected can decrease product efficacy, alter its quality, and trigger immunotoxic reactions after the administration of bio-therapeutics or cell and gene therapies.
Lentiviral Titration
Lentiviral vectors are used in gene transfer workflows during cell and gene therapy manufacturing. Viral vector titration is an important step in this process. ELISAs are commonly used to assess viral titer. HIV-1 p24 Gag is typically used as a proxy target analyte to titrate lentiviral vectors. The automated Simple Plex HIV-1 p24 Gag 72X1 immunoassay expedites your workflow and provides fast and consistent results.
