Explore Proteome Profiler™ Antibody Arrays
Discover the innovation of Proteome Profiler Antibody Arrays, cost-effective tools designed for early-stage research exploration with significant differentiating features.
This antibody array system, offering semiquantitative analysis, empowers researchers to measure over 100 proteins in a single sample. The simplicity of this method is underscored by its independence from specialized equipment, rendering multiple Western blot experiments unnecessary. Each antibody array kit comprises buffers, detection antibodies, and membranes meticulously spotted in duplicate with high-quality capture antibodies. Utilizing chemiluminescence for detection, the resulting data can be quantified with the same precision as traditional Western blots.
Select antibody arrays are also suitable for use with the LI-COR® detection systems.
Ready for a more quantitative multiplex assay? Try our Luminex® Assays.
Search Proteome Profiler Antibody Arrays
Profile Human Extracellular or Intracellular Proteins
Profile Human Extracellular or Intracellular Proteins
Use the Proteome Profiler antibody array to screen human proteins such as cytokines, cancer biomarkers, kinases, soluble receptors, ubiquitin-related proteins proteases, protease inhibitors and more!
Profile Mouse Extracellular or Intracellular Proteins
Profile Mouse Extracellular or Intracellular Proteins
Use Proteome Profiler antibody arrays to screen mouse proteins such as adipokines, cytokines, chemokines, kinases, apoptotic proteins and angiogenesis related proteins.
Profile Rat Adipokines and Cytokines
Profile Rat Adipokines and Cytokines
Profile rat adipokines and cytokines with Proteome Profiler antibody arrays.
Quick Spots Image Analysis Software
R&D Systems® now offers Quick Spots image analysis software designed specifically for your Proteome Profiler™ Arrays.
No more laborious set-up of templates in other imaging software, Quick Spots contains preloaded templates for each Proteome Profiler™ array. It knows where your results should be and provides the data in less time and with less work.
Webinar: Understanding Immunosuppressive Myeloid Cells and Immunotherapy
Advanced Prostate Cancer (PCa) can progress to lethal metastatic castration-resistant prostate cancer (mCRPC), even when treated with androgen deprivation therapy. Although immune checkpoint blockade has revolutionized the way cancer is treated, it is less effective in this context. Watch this video to see how Proteome Profiler antibody arrays were used by Dr. Xin Lu and his research team as they addressed this challenge. See how they demonstrated increased synergistic antitumor efficacy when an immune checkpoint blockade cocktail (CTLA-4 and PD1) is used in combination with PI3-kinase signaling pathway inhibitors. Find out about the role that myeloid-derived suppressor cells play in aggressive cancer.
Profiling Changes in Receptor Tyrosine Kinase Phosphorylation Using Antibody
Profiling Changes in Receptor Tyrosine Kinase Phosphorylation Using Antibody
Watch this video to learn about the principle of the Proteome Profiler antibody array. See how assays are run with this step-by-step detailed description of the method.
Antibody Array Principle
- Capture antibodies are spotted in duplicate on the nitrocellulose membrane.
- Spotted membrane arrays are incubated in a single lysate.
- Target analytes are captured by the capture antibody.
- Target analytes are detected using a biotinylated or horseradish peroxidase (HRP)-conjugated detection antibody.
- Biotinylated detection antibodies are reacted with streptavidin HRP.
- Membranes are incubated in a chemiluminescence reagent that produces a light signal proportional to the amount of antibody-target analyte complexes formed.
- Data is visualized using standard western blotting methods such as film or digital cameras.
Learn more about adipokines, chemokines and other markers by exploring our clickable signaling pathways.
Adipokines and Insulin Signaling Pathways
Embryonic and Induced Pluripotent Stem Cell Differentiation Pathways & Lineage-specific Markers
Luminex® is a trademark of Luminex Corporation.