Single Cell Protein Analysis with Milo
Meet Milo
Meet Milo
Milo™ performs Single Cell Western blot analysis on 1,000 single cells in one 4-hour experiment.
Milo Consumables
Milo Consumables
The one-stop-shop for all the consumables you need to perform Single Cell Western blot analysis on Milo, including scWest kits, chips, and buffers.
Milo Antibodies
Milo Antibodies
Milo is an open platform so you can use any antibody to probe your target. Find antibodies validated for Milo.
The Single Cell Western Blot Has Arrived
Milo is the world’s first Single Cell Western blotting platform that measures protein expression in ~1000 single cells per run using conventional Western blot antibodies. Simply load your cell suspension on a scWest chip, then Milo performs lysis, SDS-PAGE separation, and UV-induced immobilization of each single cell lysate on-chip in a matter of minutes. Probe the scWest chip with conventional Western blot antibodies and use a microarray scanner to measure ~12 proteins per cell using a variety of multiplexing strategies, or store your chip for up to 9 months for future analysis.
Discover the World of Single Cell Proteomics with Milo
Here are just a few examples of the single cell analysis studies that are made possible with Single Cell Western blot analysis on Milo.
Profile Heterogeneous Proteins and Cell Types
Profile Heterogeneous Proteins and Cell Types
Milo’s single cell heterogeneity analysis profiles heterogeneous proteins and cell types in your samples that would go undetected by traditional Western blot.
Multiplexed Single Cell Heterogeneity Analysis of Single Red Blood Cells
Single Cell Heterogeneity Analysis of the Tumor Microenvironment
Monitor Stem Cell Proteomics
Monitor Stem Cell Proteomics
Check out these App Notes to learn how Single Cell Western blotting with Milo can track and quantify single stem cell proteomics and differentiation into neural cell subtypes and cardiomyocytes:
Easier Than Single Cell Flow Cytometry
Milo is easier to use than other single cell analysis technologies like single cell flow cytometry. With Milo’s single cell analysis, you can detect proteins in single cells that escape detection by single cell flow cytometry due to their intracellular location or due to the lack of flow cytometry-validated antibodies. You can also pair Milo with fluorescence-activated cell sorting (FACS) to sort cell subtypes or live/dead cells for downstream single cell analysis on Milo.
Learn How Milo Streamlines Your Single Cell Flow Cytometry Experiments
Develop New Cell and Gene Therapies
Develop New Cell and Gene Therapies
Reveal the functional potency of viral vectors and other gene delivery systems. Measure their gene editing efficiency by monitoring target protein expression in single cells following genetic transformation to develop new cell and gene therapies.
Validate Single Cell Sequencing Data
Validate Single Cell Sequencing Data
Pair Milo’s single cell proteomics with single cell RNA sequencing workflows for complete single cell multiomic analysis. Milo provides single cell protein expression information to validate your single cell RNA seq analysis single cell protein expression data.
Publish Your Single Cell Western Blots in Premier Journals
Milo has proven its ability to provide new and profound scientific insight that traditional Western blots cannot. This fact is reflected in the rank of the journals that feature articles with Single Cell Western blot data with the vast majority (74%) of articles featuring Single Cell Western blot data in top journals with impact factors of 7 or greater including Science, Cell, Nature Methods, Nature Communications, and Cell Reports. 32% of Single Cell Western blot publications are in journals considered here to be in the premier category with an impact factor greater than 21.
Learn How Single Cell Western Blot is a Proven, High-Impact Technology
Customer Success Stories
Postdoctoral Fellow, MIT
Postdoctoral Associate, Duke University School of Medicine
Research Associate, Memorial Sloan Kettering Cancer Center
Postdoctoral associate, Weill Cornell Medicine
Requirements and Compatibility
|
Sample type |
Suspension containing >10,000 cells |
|---|---|
|
Cell diameter |
7-25 µm in suspension |
|
Cell type |
Mammalian cells; globular in suspension and unfixed |
|
Antibody requirement |
Standard unlabeled primaries and fluorescent secondaries |
|
Other equipment needed |
Open-format fluorescence microarray scanner capable of 5 µm resolution |
Performance and Specifications
|
Typical cell dilutions |
Yield capture and analysis of 1,000-2,000 cells per scWest chip |
|---|---|
|
Molecular weight (MW) range |
15-175 kDa |
|
MW resolution |
10% difference in distinct spectral channels, as low as 30% differences in same spectral channel |
|
Typical target multiplexing |
Up to four proteins per cell by spectral and size-based multiplexing |
|
Workflow time |
4-6 hours |
Single Cell Western Blot Applications
Cancer and Immuno-Oncology
Cancer and Immuno-Oncology
Transform your cancer & immuno-oncology research by utilizing next-generation Western blotting tools to give you richer views into your samples.
Cell and Gene Therapy
Next-generation analytical instrumentation to help improve and accelerate your cell and gene therapy workflows.
Cell Signaling
Uncover phospho-protein heterogeneity and quantify how phosphorylation varies across stimulated and unstimulated cell populations.
Neuroscience
Neuroscience
Increase your sensitivity and multiplexing capabilities with neural samples using automated Western blotting systems.