• Different formats of organoid-qualified Cultrex Basement Membrane Extracts are available to promote robust cell growth, viability, and culture consistency

• Our latest, most advanced Cultrex UltiMatrix RGF Basement Membrane Extract has been optimized to improve the performance and consistency of spheroid, organoid, and stem cell cultures

• Reduced growth factor formulations provide more defined culture systems

• Quality controlled for lot-to-lot performance consistency

• We offer an extensive catalog of proteins commonly used for organoid culture, including proteins that are difficult to express and purify such as R-Spondin-1, Noggin, and Wnt-3a

• All of our recombinant proteins are rigorously tested to ensure high levels of bioactivity and lot-to-lot consistency, and low endotoxin levels

• Our proteins are highly cited in organoid-related scientific publications 

• From basic research to preclinical and clinical applications, we offer research-grade, Animal-free RUO, and Animal-free GMP-grade cytokines to meet every researcher's need

• Our recombinant proteins can be ordered in bulk at discounted prices and custom protein services are also available

• A large selection of small molecules that are commonly used as 3-D growth matrix components and additives for long-term organoid growth are available

• Our catalog includes the ALK kinase inhibitor, A 83-01, the highly selective GSK-3 inhibitor CHIR 99021, the potent ROCK inhibitor, Y-27632 dihydrochloride, the p38 MAPK inhibitor, SB 202190, and many more

• We offer a variety of different formats of base media for organoid cultures, including media with or without glucose, L-glutamine, HEPES, NEAA, nucleosides, phenol red, or sodium pyruvate content

• We develop and manufacture serum-free media supplements to support organoid cultures

• Our catalog includes cryopreservation media, which are optimized to increase cell recovery and viability

• Our New ExCellerate GMP iPSC Expansion Medium is completely animal-free and does not require the addition of growth factors

• Our catalog includes over 425,000 R&D Systems™ and Novus Biologicals™ antibodies for more than 25 species

• We offer both unlabeled and labeled primary antibodies with 20 different potential fluorescent conjugates

• Our primary antibodies are validated for one or more of up to 15 applications

• We offer both conjugated and unconjugated secondary antibodies and a range of isotype control antibodies

• Our antibodies come in a variety of size options, including bulk and sample sizes to ensure that you can find what you need for your research

• Utilize this technology to spatially map and quantify specific target mRNAs in intact cells and tissues

• The assay can be combined with immunohistochemistry or immunofluorescence on the same slide to investigate mRNA and protein co-expression

• Up to 12 RNA targets can be detected simultaneously using the RNAscope HiPlex assay

• Catalog probes are available for over 23,000 targets in 140+ species and can also be made-to-order

• RNAscope ISH Assays are cited in over 2,600 publications across multiple different research areas

This handbook highlights some of the notable publications that have used organoids to investigate questions about development, disease, or drug safety. Additionally, it includes recipes and links to the key reagents that our own in-house scientists have used to establish the culture conditions for different types of organoids.

Establishing and optimizing the appropriate conditions for culturing different types of organoids can be technically challenging. Read this application note to learn about the importance of growth factor bioactivity and reagent consistency for achieving robust, reproducible organoid growth.

Organoids are emerging as powerful new platforms for exploring human organ development, modeling disease, screening for drug toxicity, and investigating personalized medicine. Request this poster to decorate your lab with some of the visually stunning organoid images that have been generated by cell and gene therapy scientists at Bio-Techne.

Watch Bio-Techne’s Virtual Organoid Symposium from May 2020 to learn more about how leading scientists are utilizing organoids in their research. Presenters include Mr. Jens Puschof and Dr. Talya Dayton from Dr. Hans Clevers lab at the Hubrecht Institute in the Netherlands, and Dr. Robert Barrett from Cedars Sinai Medical Center in Los Angeles, CA.

Browse our collection of products for cell culture. We offer a comprehensive range of reagents to promote robust cell growth, including media and supplements, basement membrane extracts, recombinant proteins, and custom cell culture services.

Minimize variability in your stem cell experiments and ensure high performance by using our stem cell culture reagents. From isolation and culture to verification, reprogramming, differentiation, and characterization, we have products that you need for your stem cell work.