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BMP-15/GDF-9B Antibody (BMP15/4263)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-07552

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Human

Applications

ELISA, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # BMP15/4263

Concentration

0.2 mg/ml

Product Specifications

Immunogen

Recognizes sequence SAEVTASSSKHSGPENNQC on the C-terminus of human BMP-15/GDF-9B (Uniprot: O95972)

Localization

Cytoplasmic (secreted)

Specificity

BMP15, also known as GDF9B, plays a crucial role in the regulation of fertility. Clone BMP15/4263 can be used to detect BMP15 expression and biosynthesis in various carcinoma progression studies.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

45 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP3-08876)

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.

Applications for BMP-15/GDF-9B Antibody (BMP15/4263)

Application
Recommended Usage

Western Blot

1-2 ug/ml
Application Notes
ELISA: For coating, order antibody without BSA).
Optimal dilution for a specific application should be determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS with 0.05% BSA

Preservative

0.05% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: BMP-15/GDF-9B

BMPs are secreted signaling molecules that comprise a subfamily of the TGF-beta superfamily and were originally identified as regulators of cartilage and bone formation. There are at least 20 structurally and functionally related BMPs, most of which play roles in embryogenesis and morphogenesis of various tissues and organs. Biologically active BMPs are usually homodimers containing a characteristic cysteine knot structure. Heterodimers, BMP-2/BMP-7 and BMP-4/BMP-7 have also been suggested to exist and function in vivo. They are more potent inducers of bone formation than their respective homodimers. In addition, heterodimers, but not homodimers, are ventral mesoderm inducers. Heterodimer activity may be mediated by a different or additional receptor subtype.

Decapentaplegic (Dpp) is one of at least five TGF-beta superfamily ligands identified in the Drosophila genome. Dpp, a functional ortholog of mammalian BMP-2 and BMP-4, is a morphogen and plays an essential role in Drosophila development. Dpp regulates embryonic dorsal-ventral polarity and is required for gut morphogenesis and outgrowth and patterning of imaginal disks.

Long Name

Bone Morphogenetic Protein 15

Alternate Names

BMP15, GDF-9B

Gene Symbol

BMP15

UniProt

Additional BMP-15/GDF-9B Products

Product Documents for BMP-15/GDF-9B Antibody (BMP15/4263)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for BMP-15/GDF-9B Antibody (BMP15/4263)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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