Canine CCL3/MIP-1 alpha Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB7370

R&D Systems, part of Bio-Techne
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MAB7370
MAB7370-SP
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Key Product Details

Species Reactivity

Canine

Applications

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # 750521

View all CCL3/MIP-1 alpha Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant canine CCL3/MIP-1 alpha
Ser23-Ala92
Accession # Q68A92

Specificity

Detects canine CCL3/MIP-1 alpha in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant canine CCL4/MIP-1 beta or recombinant CCL3/MIP-1 alpha from human, mouse, rat, or cotton rat is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Scientific Data Images for Canine CCL3/MIP-1 alpha Antibody

Detection of Canine CCL3/MIP-1a antibody by Western Blot.

Detection of Canine CCL3/MIP‑1 alpha by Western Blot.

Western blot shows lysates of A-72 canine fibroma cell line. PVDF membrane was probed with 0.1 µg/mL of Mouse Anti-Canine CCL3/MIP-1a Monoclonal Antibody (Catalog # MAB7370) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for CCL3/MIP-1a at approximately 5 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 10.

Applications for Canine CCL3/MIP-1 alpha Antibody

Application
Recommended Usage

Western Blot

0.1 µg/mL
Sample: A‑72 canine fibroma cell line
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CCL3/MIP-1 alpha

CCL3, also known as macrophage inflammatory protein 1 alpha (MIP-1 alpha) and LD78, is a member of the beta or CC subfamily of chemokines and is closely related to CCL4/MIP-1 beta. Chemokines comprise a large family of small secreted proteins that are involved in immune and inflammatory responses. CCL3 expression can be induced in a variety of hematopoietic cells, fibroblasts, smooth muscle cells, and epithelial cells (1). Mature canine CCL3 shares 66-67%amino acid sequence identity with human, mouse, and rat CCL3. CCL3 is an approximately 8 kDa chemokine that forms complexes with sulfated proteoglycans (2, 3). In a reversible process, CCL3 associates into noncovalently-linked dimers which then form tetramers and high molecular weight polymers (4, 5). These complexes of CCL3 are protected from proteolytic digestion by insulin degrading enzyme (IDE) which can cleave the monomeric chemokine (5). CCL3 exerts its biological functions through interactions with CCR1, CCR3, and CCR5 (1). It is cleared from the extracellular space by internalization via the decoy chemokine receptor D6 (6). CCL3 promotes the chemoattraction, adhesion to activated vascular endothelium, and cellular activation of many hematopoietic cell types including activated T cells, NK cells, neutrophils, monocytes, immature dendritic cells, and eosinophils (1, 7‑9). CCL3 is also known as stem cell inhibitor (SCI) and can inhibit the proliferation of hematopoietic progenitor cells (2). CCL3 bioactivity contributes to tumor metastasis and the inflammatory components of viral infection, rheumatoid arthritis, and hepatitis (10‑13), although it also can suppress the replication of HIV (14). CCL3 additionally promotes hyperalgesia by sensitizing sensory neurons to TRPV1‑mediated noxious stimulation (15).

References

  1. Menten, P. et al. (2002) Cytokine Growth Factor Rev. 13:455.
  2. Graham, G.J. et al. (1990) Nature 344:442.
  3. Wagner, L. et al. (1998) Nature 391:908.
  4. Graham, G.J. et al. (1994) J. Biol. Chem. 269:4974.
  5. Ren, M. et al. (2010) EMBO J. 29:3952.
  6. Weber, M. et al. (2004) Mol. Biol. Cell 15:2492
  7. Taub, D.D. et al. (1993) Science 260:355.
  8. Bernardini, G. et al. (2008) Blood 111:3626.
  9. Lee, S.C. et al. (2000) J. Immunol. 164:3392.
  10. Wu, Y. et al. (2008) J. Immunol. 181:6384.
  11. Cook, D.N. et al. (1995) Science 269:1583.
  12. Chintalacharuvu, S.R. et al. (2005) Immunol. Lett. 100:202.
  13. Ajuebor, M.N. et al. (2004) Eur. J. Immunol. 34:2907.
  14. Cocchi, F. et al. (1995) Science 270:1811.
  15. Zhang, N. et al. (2005) Proc. Natl. Acad. Sci. 102:4536.

Alternate Names

G0S19-1, LD78a, MIP-1 alpha, MIP1 alpha, MIP1A, PAT 464.1, SCYA3, SIS-beta

Entrez Gene IDs

6348 (Human); 20302 (Mouse); 25542 (Rat); 448787 (Canine); 102136134 (Cynomolgus Monkey)

Gene Symbol

CCL3

UniProt

Q68A92

Additional CCL3/MIP-1 alpha Products

Product Documents for Canine CCL3/MIP-1 alpha Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Canine CCL3/MIP-1 alpha Antibody

For research use only

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