Canine IL-18/IL-1F4 Antibody
R&D Systems, part of Bio-Techne | Catalog # AF2924
Key Product Details
Species Reactivity
Canine
Applications
Immunocytochemistry, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
E. coli-derived recombinant canine IL‑18/IL‑1F4
Tyr37-Ser193
Accession # Q9XSR0
Tyr37-Ser193
Accession # Q9XSR0
Specificity
Detects canine IL‑18/IL‑1F4 in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 50% cross-reactivity with recombinant porcine IL-18 is observed, 20% cross-reactivity with recombinant rhesus macaque IL-18 is observed, 15% cross-reactivity with recombinant human IL-18 is observed, and 5% cross-reactivity with recombinant mouse IL-18 and recombinant rat IL-18 is observed.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Canine IL-18/IL-1F4 Antibody
IL‑18/IL‑1F4 in Canine PBMCs.
IL-18/IL-1F4 was detected in immersion fixed canine peripheral blood mononuclear cells (PBMCs) using Goat Anti-Canine IL-18/IL-1F4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2924) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm and cell secretions. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.Applications for Canine IL-18/IL-1F4 Antibody
Application
Recommended Usage
Immunocytochemistry
5-15 µg/mL
Sample: Immersion fixed canine peripheral blood mononuclear cells
Sample: Immersion fixed canine peripheral blood mononuclear cells
Western Blot
0.1 µg/mL
Sample: Recombinant Canine IL-18/IL-1F4
Sample: Recombinant Canine IL-18/IL-1F4
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: IL-18/IL-1F4
Long Name
Interleukin 18
Alternate Names
IGIF, IL-1F4, IL-1g, IL18
Gene Symbol
IL18
UniProt
Additional IL-18/IL-1F4 Products
Product Documents for Canine IL-18/IL-1F4 Antibody
Product Specific Notices for Canine IL-18/IL-1F4 Antibody
For research use only
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