Caspase-10/FLICE2 Antibody (JE59-42) - Pro

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-32856

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-32856
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # JE59-42

Concentration

1 mg/ml

View all Caspase-10 Antibodies »

Product Specifications

Immunogen

Synthetic peptide. (Uniprot: Q92851)

Localization

Cytoplasm, cytosol, ripoptosome.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

59 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Caspase-10/FLICE2 Antibody (JE59-42) - Pro

Caspase-10/FLICE2 Antibody (JE59-42) - Pro

Western Blot: Caspase-10/FLICE2 Antibody (JE59-42) - Pro [NBP3-32856]

Western Blot: Caspase-10/FLICE2 Antibody (JE59-42) - Pro [NBP3-32856] - Western blot analysis of Caspase-10/FLICE2 on different lysates with Rabbit anti-Caspase-10/FLICE2 antibody (NBP3-32856) at 1/1,000 dilution.

Lane 1: HepG2 cell lysate
Lane 2: A431 cell lysate
Lane 3: K-562 cell lysate

Lysates/proteins at 20 ug/Lane.

Predicted band size: 59 kDa
Observed band size: 59 kDa

Exposure time: 3 minutes;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32856) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.
Caspase-10/FLICE2 Antibody (JE59-42) - Pro

Immunohistochemistry: Caspase-10/FLICE2 Antibody (JE59-42) - Pro [NBP3-32856]

Immunohistochemistry: Caspase-10/FLICE2 Antibody (JE59-42) - Pro [NBP3-32856] - Immunohistochemical analysis of paraffin-embedded human breast cancer tissue with Rabbit anti-Caspase-10/FLICE2 antibody (NBP3-32856) at 1/2,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32856) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Applications for Caspase-10/FLICE2 Antibody (JE59-42) - Pro

Application
Recommended Usage

Immunohistochemistry-Paraffin

1:2000

Western Blot

1:1000

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

1*TBS (pH7.4), 0.05% BSA and 40% Glycerol

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Caspase-10

Apoptosis, or programmed cell death, is a common property of all multicellular organisms. The current dogma of apoptosis suggests that the components of the core cell-death machinery are integral to cells and widely conserved across species. Caspases, a family of cysteinyl aspartate-specific proteases, are integral components of the cell death machinery (reviewed in Siegal, 2006; and Lavrik et al, 2005). They play a central role in the initiation and execution of apoptotic cell death and in inflammation. Caspases are typically divided into 3 major groups, depending on the structure of their prodomain and their function. Group 1: inflammatory caspases (caspases 1, 4, 5, 11, 12, 14). Group II: initiator of apoptosis caspases (caspases 2, 8, 9). Group II: effector caspases (caspases 3, 6, 7). Caspases are constitutively expressed in almost all cell types as inactive proenzymes (zymogens: enzyme precursors which require a biochemical change to become active enzymes) that are processed and activated in response to a variety of pro-apoptotic or inflammatory stimuli. The procaspases (32-56 kDa) contain four domains: an N-terminal prodomain (2-25 kDa), a large subunit (p20: 17-21 kDa), a small subunit (p10: 10-13 kDa) and a short linker region between the large and small subunits. Caspase activation involves proteolytic processing of the proenzyme at specific aspartate residues between the domains. This results in removal of the prodomain as well as the linker region and formation of a heterodimer containing one large and one small subunit (p20-p10). The active caspase is a tetramer composed of two heterodimers (p202-p102). Active caspases mediate cell death and inflammation through cleavage of particular cellular substrates that are involved in these processes. Caspase-10 is a 455 amino acid protein. However, caspase-10 produces, by alternative splicing, at least 12 different transcripts, all with introns, putatively encoding at least 11 different protein isoforms. For example, human caspase-10a is a 479 amino acid (aa) protein, and human caspase-10d is a 522 aa protein. The transcripts appear to differ by truncation of the end, presence or absence of 6 cassette exons, or common exons with different boundaries, because an internal intron is not always spliced out. Users are encoraged to go to the NCBI ACE View Data Base for more information. http://www.ncbi.nih.gov/IEB/Research/Acembly/ Caspase-10 gene mutations or polymorphisms have been associated with a number of diseases including type II autoimmune lymphoproliferative syndrome, non-Hiodgkin's lymphoma, and gastric cancer. It is thought that some mutations of the caspase-10 gene may lead to the loss of its apoptotic function, thereby contributing to the pathogenesis of some diseases. Caspase-10 may be observed on Western blots at varying apparent molecular weights depending on isoform, truncation, or cleavage forms present. Recognizes caspase-10 forms that contain the peptide immunogen sequence (QPAFTLRKKLVFPVPLDALSI).

Alternate Names

CASP10, Caspase10, FLICE2, Mch4

Gene Symbol

CASP10

Additional Caspase-10 Products

Product Documents for Caspase-10/FLICE2 Antibody (JE59-42) - Pro

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Caspase-10/FLICE2 Antibody (JE59-42) - Pro

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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