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CD161 Antibody (10/78) - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NB100-65297

Novus Biologicals, part of Bio-Techne
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NB100-65297
NB100-65297SS
Conjugate
Catalog #

Key Product Details

Species Reactivity

Validated:

Rat

Cited:

Rat

Applications

Validated:

Flow Cytometry, Immunohistochemistry, Immunohistochemistry-Frozen, Immunoprecipitation

Cited:

Flow Cytometry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 10/78

Format

BSA Free

Concentration

1.0 mg/ml

Product Specifications

Immunogen

Purified splenic NK cells from the LEW rat strain.

Specificity

Recognizes the rat CD161 protein (also known as NKR-PI) a 60kD glycoprotein expressed on rat NK cells and T cell subpopulations. This competes with clone 3.2.3 for binding to antigen.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for CD161 Antibody (10/78) - BSA Free

Flow Cytometry: CD161 Antibody (10/78) - BSA Free [NB100-65297]

Flow Cytometry: CD161 Antibody (10/78) - BSA Free [NB100-65297]

Flow Cytometry: CD161 Antibody (10/78) [NB100-65297] - Rat splenocytes were stained with Anti-Rat CD3 APC-conjugated Monoclonal Antibody and either (A) Mouse Anti-Rat CD161 Monoclonal Antibody (Catalog# NB100-65297) or (B) Mouse IgG1 Isotype control (Catalog# MAB002) followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog# F0102B). View our protocol for Staining Membrane-associated Proteins.

Applications for CD161 Antibody (10/78) - BSA Free

Application
Recommended Usage

Flow Cytometry

1:10-1:1000

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Frozen

1:10-1:500

Immunoprecipitation

1:10-1:500
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 1 review rated 1 using NB100-65297 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: CD161

CD161, also known as KLRB1, NK1.1, and NKR-P1A is a 40 kDa type II transmembrane glycoprotein that contains one C-type lectin domain in its extracellular region. CD161 is expressed as a disulfide-linked dimer on the surface of Th17 cells and NK cells as well as on subsets of CD1-restricted T cells, intestinal NT cells, peripheral memory T cells, monocytes, and dendritic cells. It binds to OCIL/CLEC2d, leading to an inhibition of NK cell-mediated cytotoxicity and IFN-gamma secretion. Alternatively, CD161 can enhance TCR activation to CD1d ligation. CD161+ cell populations are depleted in ulcerative colitis, Grave's disease, and AIDS, although CD161+ T cells are activated during asthmatic attacks. Additional related proteins are expressed in mouse but not human: the inhibitory NKR-P1B and D, and the stimulatory NKR-P1A, C, and F.

Alternate Names

CD161, CLEC5B, KLRB1, Ly59, NKR, NKRP1, NKRP1A

Entrez Gene IDs

683758 (Rat)

Gene Symbol

KLRB1

UniProt

Additional CD161 Products

Product Documents for CD161 Antibody (10/78) - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for CD161 Antibody (10/78) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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