CD44 Antibody (156-3C11)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-34327

Clone 156-3C11 was used by HLDA to establish CD designation.
Novus Biologicals, part of Bio-Techne
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NBP2-34327-0.1mg
NBP2-34327-0.02mg
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Key Product Details

Species Reactivity

Validated:

Human, Baboon, Monkey

Cited:

Human

Applications

Validated:

Flow (Cell Surface), Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

Flow Cytometry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 156-3C11

Concentration

0.2 mg/ml

View all CD44 Antibodies »

Product Specifications

Immunogen

Stimulated human leukocytes

Reactivity Notes

Green Monkey

Localization

Cell surface

Specificity

Recognizes a cell surface glycoprotein of 80-95kDa (CD44) on lymphocytes, monocytes, and granulocytes (Leucocyte Typing Workshop V). Its epitope is resistant to digestion by trypsin and chymotrypsin. This monoclonal antibody selectively interferes with lymphocyte binding to lymph node, mucosal and synovial endothelium. The CD44 family of glycoproteins exists in a number of variant isoforms, the most common being the standard 85-95kDa or hematopoietic variant (CD44s). Higher molecular weight isoforms are described in epithelial cells (CD44v), which are believed to function in intercellular adhesion and stromal binding. CD44 immunostaining is commonly used for the discrimination of urothelial transitional cell carcinoma in-situ from non-neoplastic changes in the urothelium.

Marker

Cancer Stem Cell Marker

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Description

200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP2-34520)

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.

Scientific Data Images for CD44 Antibody (156-3C11)

Western Blot: CD44 Antibody (156-3C11) [NBP2-34327]

Western Blot: CD44 Antibody (156-3C11) [NBP2-34327]

Western Blot: CD44 Antibody (156-3C11) [NBP2-34327] - Western Blot Analysis of HeLa cell lysate using CD44 Antibody (156-3C11).
Immunocytochemistry/ Immunofluorescence: CD44 Antibody (156-3C11) [NBP2-34327]

Immunocytochemistry/ Immunofluorescence: CD44 Antibody (156-3C11) [NBP2-34327]

Immunocytochemistry/Immunofluorescence: CD44 Antibody (156-3C11) [NBP2-34327] - Confocal immunofluorescence image of HeLa cells using CD44 Antibody (156-3C11) Green (CF488) and Red Dot is used to label the nuclei Red.
Immunohistochemistry-Paraffin: CD44 Antibody (156-3C11) [NBP2-34327]

Immunohistochemistry-Paraffin: CD44 Antibody (156-3C11) [NBP2-34327]

Immunohistochemistry-Paraffin: CD44 Antibody (156-3C11) [NBP2-34327] - Formalin-paraffin human breast carcinoma stained with CD44 MAb (156-3C11)
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Applications for CD44 Antibody (156-3C11)

Application
Recommended Usage

Flow Cytometry

2-4 ug/million cells

Immunocytochemistry/ Immunofluorescence

2-4 ug/ml

Immunohistochemistry-Paraffin

1-2 ug/ml

Western Blot

1-2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS with 0.05% BSA

Preservative

0.05% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: CD44

CD44 represents a family of type I transmembrane glycoproteins expressed on many cell types such as B cells, monocytes, macrophages, and variable subsets of thymocytes and peripheral T cells. CD44 primarily functions as an adhesion molecule. It has been implicated in various processes, such as hematopoiesis, lymphocyte homing, leukocyte activation, tumor metastasis and development. The KM201 monoclonal antibody reacts with an epitope very close to the hyaluronate binding domain on the CD44 molecule. KM201 can inhibit hyaluronate-dependent cell aggregation, prevent lympho-hemopoiesis in both Dexter and Whitlock-Witte Cultures, prevent the earliest intrathymic precursors from homing to the thymus, and costimulate the activation of freshly purified splenic CD4+ T cells.1-6

Alternate Names

CD44, ECMR-III, HCAM, HCELL, LHR, MDU2, MDU3, MIC4, MUTCH-I, Pgp1

Gene Symbol

CD44

UniProt

P16070

Additional CD44 Products

Product Documents for CD44 Antibody (156-3C11)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for CD44 Antibody (156-3C11)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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