CD44 Antibody (156-3C11) - IHC-Prediluted
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-45346
Clone 156-3C11 was used by HLDA to establish CD designation.
![Immunohistochemistry-Paraffin: CD44 Antibody (156-3C11) - IHC-Prediluted [NBP2-45346]](https://resources.bio-techne.com/images/products/CD44-Antibody-156-3C11-IHC-Prediluted-Immunohistochemistry-Paraffin-NBP2-45346-img0005.jpg "Immunohistochemistry-Paraffin: CD44 Antibody (156-3C11) - IHC-Prediluted [NBP2-45346]")
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Human, Baboon, Monkey
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2a Kappa Clone # 156-3C11
Format
IHC-Prediluted
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Stimulated human leukocytes
Reactivity Notes
Green Monkey
Localization
Cell Surface
Specificity
Recognizes a cell surface glycoprotein of 80-95kDa (CD44) on lymphocytes, monocytes, and granulocytes (Leucocyte Typing Workshop V). Its epitope is resistant to digestion by trypsin and chymotrypsin. This monoclonal antibody selectively interferes with lymphocyte binding to lymph node, mucosal and synovial endothelium. The CD44 family of glycoproteins exists in a number of variant isoforms, the most common being the standard 85-95kDa or hematopoietic variant (CD44s). Higher molecular weight isoforms are described in epithelial cells (CD44v), which are believed to function in intercellular adhesion and stromal binding. CD44 immunostaining is commonly used for the discrimination of urothelial transitional cell carcinoma in-situ from non-neoplastic changes in the urothelium.
Marker
Cancer Stem Cell Marker
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2a Kappa
Description
The prediluted antibody does not require any mixing, dilution, reconstitution, or titration; the antibody is ready-to-use and optimized for staining.
Scientific Data Images for CD44 Antibody (156-3C11) - IHC-Prediluted
Immunohistochemistry-Paraffin: CD44 Antibody (156-3C11) - IHC-Prediluted [NBP2-45346]
Immunohistochemistry-Paraffin: CD44 Antibody (156-3C11) - IHC-Prediluted [NBP2-45346] - Formalin-fixed, paraffin-embedded human tonsil stained with CD44 MAb (156-3C11)![Immunohistochemistry-Paraffin: CD44 Antibody (156-3C11) - IHC-Prediluted [NBP2-45346]](https://resources.bio-techne.com/images/products/CD44-Antibody-156-3C11-IHC-Prediluted-Immunohistochemistry-Paraffin-NBP2-45346-img0004.jpg "Immunohistochemistry-Paraffin: CD44 Antibody (156-3C11) - IHC-Prediluted [NBP2-45346]")
Immunohistochemistry-Paraffin: CD44 Antibody (156-3C11) - IHC-Prediluted [NBP2-45346]
Immunohistochemistry-Paraffin: CD44 Antibody (156-3C11) - IHC-Prediluted [NBP2-45346] - Formalin-paraffin human breast carcinoma stained with CD44 MAb (156-3C11)Applications for CD44 Antibody (156-3C11) - IHC-Prediluted
Application
Recommended Usage
Immunohistochemistry-Paraffin
0.25 - 0.5 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Optimal dilution for a specific application should be determined.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
10 mM PBS with 0.05% BSA
Format
IHC-Prediluted
Preservative
0.05% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C.
Background: CD44
Alternate Names
CD44, ECMR-III, HCAM, HCELL, LHR, MDU2, MDU3, MIC4, MUTCH-I, Pgp1
Gene Symbol
CD44
Additional CD44 Products
Product Documents for CD44 Antibody (156-3C11) - IHC-Prediluted
Product Specific Notices for CD44 Antibody (156-3C11) - IHC-Prediluted
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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