CD44 Antibody (156-3C11) - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-34520
Clone 156-3C11 was used by HLDA to establish CD designation.
![Immunohistochemistry-Paraffin: CD44 Antibody (156-3C11) - Azide and BSA Free [NBP2-34520]](https://resources.bio-techne.com/images/products/CD44-Antibody-156-3C11-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-34520-img0004.jpg "Immunohistochemistry-Paraffin: CD44 Antibody (156-3C11) - Azide and BSA Free [NBP2-34520]")
![Western Blot: CD44 Antibody (156-3C11)Azide and BSA Free [NBP2-34520]](https://resources.bio-techne.com/images/products/CD44-Antibody-156-3C11-Azide-and-BSA-Free-Western-Blot-NBP2-34520-img0003.jpg "Western Blot: CD44 Antibody (156-3C11)Azide and BSA Free [NBP2-34520]")
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Human, Baboon, Monkey
Applications
CyTOF-ready, Flow (Cell Surface), Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2a Kappa Clone # 156-3C11
Format
Azide and BSA Free
Concentration
1.0 mg/ml
Product Specifications
Immunogen
Stimulated human leukocytes
Reactivity Notes
Green Monkey
Localization
Cell surface
Specificity
Recognizes a cell surface glycoprotein of 80-95kDa (CD44) on lymphocytes, monocytes, and granulocytes (Leucocyte Typing Workshop V). Its epitope is resistant to digestion by trypsin and chymotrypsin. This monoclonal antibody selectively interferes with lymphocyte binding to lymph node, mucosal and synovial endothelium. The CD44 family of glycoproteins exists in a number of variant isoforms, the most common being the standard 85-95kDa or hematopoietic variant (CD44s). Higher molecular weight isoforms are described in epithelial cells (CD44v), which are believed to function in intercellular adhesion and stromal binding. CD44 immunostaining is commonly used for the discrimination of urothelial transitional cell carcinoma in-situ from non-neoplastic changes in the urothelium.
Marker
Cancer Stem Cell Marker
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2a Kappa
Description
1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP2-34327).
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Scientific Data Images for CD44 Antibody (156-3C11) - Azide and BSA Free
Western Blot: CD44 Antibody (156-3C11)Azide and BSA Free [NBP2-34520]
Western Blot: CD44 Antibody (156-3C11) - Azide and BSA Free [NBP2-34520] - Western Blot Analysis of HeLa cell lysate using CD44 Antibody (156-3C11).![Immunocytochemistry/ Immunofluorescence: CD44 Antibody (156-3C11) - Azide and BSA Free [NBP2-34520]](https://resources.bio-techne.com/images/products/CD44-Antibody-156-3C11-Azide-and-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP2-34520-img0002.jpg "Immunocytochemistry/ Immunofluorescence: CD44 Antibody (156-3C11) - Azide and BSA Free [NBP2-34520]")
Immunocytochemistry/ Immunofluorescence: CD44 Antibody (156-3C11) - Azide and BSA Free [NBP2-34520]
Immunocytochemistry/Immunofluorescence: CD44 Antibody (156-3C11) - Azide and BSA Free [NBP2-34520] - Confocal immunofluorescence image of HeLa cells using CD44 Antibody (156-3C11) Green (CF488) and Red Dot is used to label the nuclei Red.![Immunohistochemistry-Paraffin: CD44 Antibody (156-3C11) - Azide and BSA Free [NBP2-34520]](https://resources.bio-techne.com/images/products/CD44-Antibody-156-3C11-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-34520-img0005.jpg "Immunohistochemistry-Paraffin: CD44 Antibody (156-3C11) - Azide and BSA Free [NBP2-34520]")
Immunohistochemistry-Paraffin: CD44 Antibody (156-3C11) - Azide and BSA Free [NBP2-34520]
Immunohistochemistry-Paraffin: CD44 Antibody (156-3C11) - Azide and BSA Free [NBP2-34520] - Formalin-fixed, paraffin-embedded human tonsil stained with CD44 MAb (156-3C11)Applications for CD44 Antibody (156-3C11) - Azide and BSA Free
Application
Recommended Usage
Flow Cytometry
0.5-1ug/million cells
Immunocytochemistry/ Immunofluorescence
0.5-1ug/ml
Immunohistochemistry-Paraffin
0.5-1ug/ml
Western Blot
0.5-1ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Optimal dilution for a specific application should be determined.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
10 mM PBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20 to -80C. Avoid freeze-thaw cycles.
Background: CD44
Alternate Names
CD44, ECMR-III, HCAM, HCELL, LHR, MDU2, MDU3, MIC4, MUTCH-I, Pgp1
Gene Symbol
CD44
Additional CD44 Products
Product Documents for CD44 Antibody (156-3C11) - Azide and BSA Free
Product Specific Notices for CD44 Antibody (156-3C11) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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