CD59 Antibody (YTH53.1)
Novus Biologicals, part of Bio-Techne | Catalog # NB100-65537
Clone YTH53.1 was used by HLDA to establish CD designation.
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Human
Applications
Flow Cytometry, Functional, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Rat IgG2B Clone # YTH53.1
Concentration
1.0 mg/ml
Product Summary for CD59 Antibody (YTH53.1)
Immunogen
Human Peripheral Blood T cells
Localization
Cell membrane; lipid anchor; GPI anchor.
Specificity
NB100-65537 recognizes the human CD59 cell surface antigen, an 18-20kD GPI linked glycoprotein broadly expressed by human leucocytes and erythrocytes. Clone YTH53.1 has been shown to block the normal function of CD59. Removal of sodium azide is recommended prior to use in functional assays. CD59 is expressed by erythrocytes, and therefore staining in whole blood systems requires a higher concentration of antibody.
Clonality
Monoclonal
Host
Rat
Isotype
IgG2B
Scientific Data Images for CD59 Antibody (YTH53.1)
Immunohistochemistry-Paraffin: CD59 Antibody (YTH53.1) [NB100-65537] Immunoperoxidase staining of formalin fixed, paraffin embedded human placenta with Rat anti Human CD59
![Flow Cytometry: CD59 Antibody (YTH53.1) - BSA Free [NB100-65537]](https://resources.bio-techne.com/images/products/CD59-Antibody-YTH53-1-Flow-Cytometry-NB100-65537-img0001.jpg "Flow Cytometry: CD59 Antibody (YTH53.1) - BSA Free [NB100-65537]")
Flow Cytometry: CD59 Antibody (YTH53.1) - BSA Free [NB100-65537]
Flow Cytometry: CD59 Antibody (YTH53.1) [NB100-65537] - Staining of human peripheral blood granulocytes with RAT ANTI HUMAN CD59 (NB100-65537).Applications for CD59 Antibody (YTH53.1)
Application
Recommended Usage
Flow Cytometry
1:50 - 1:100
Immunohistochemistry
1:10 - 1:500
Immunohistochemistry-Frozen
1:10 - 1:500
Immunohistochemistry-Paraffin
1:5 - 1:10
Western Blot
1:100 - 1:2000
Application Notes
This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate buffer pH 6.0 is recommended for this purpose. This product contains sodium azide, removal by dialysis is recommended prior to use in functional assays.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein G purified
Formulation
PBS
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: CD59
Additional CD59 Products
Product Documents for CD59 Antibody (YTH53.1)
Product Specific Notices for CD59 Antibody (YTH53.1)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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