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CD59 Antibody (YTH53.1) [PE]

Novus Biologicals, part of Bio-Techne | Catalog # NB100-65537PE

Clone YTH53.1 was used by HLDA to establish CD designation.
Novus Biologicals, part of Bio-Techne
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NB100-65537PE
Conjugate
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Key Product Details

Species Reactivity

Validated:

Human

Applications

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Western Blot

Label

PE (Excitation = 488 nm, Emission = 575 nm)

Antibody Source

Monoclonal Rat IgG2B Clone # YTH53.1

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Product Summary for CD59 Antibody (YTH53.1) [PE]

Immunogen

Human Peripheral Blood T cells

Localization

Cell membrane; lipid anchor; GPI anchor.

Specificity

NB100-65537 recognizes the human CD59 cell surface antigen, an 18-20kD GPI linked glycoprotein broadly expressed by human leucocytes and erythrocytes. Clone YTH53.1 has been shown to block the normal function of CD59. Removal of sodium azide is recommended prior to use in functional assays. CD59 is expressed by erythrocytes, and therefore staining in whole blood systems requires a higher concentration of antibody.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2B

Description

This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Applications for CD59 Antibody (YTH53.1) [PE]

Application
Recommended Usage

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Frozen

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Paraffin

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Optimal dilution of this antibody should be experimentally determined.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark.

Background: CD59

CD59, an LY6 like protein expressed in human lymphoid cells, regulates the action of the complement membrane attack complex on homologous cells.

Alternate Names

CD59, HRF20, MACIF, MIC11, Protectin

Gene Symbol

CD59

Additional CD59 Products

Product Documents for CD59 Antibody (YTH53.1) [PE]

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for CD59 Antibody (YTH53.1) [PE]

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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