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Key Product Details

Species Reactivity

Human

Applications

CyTOF-ready, Flow Cytometry, Immunocytochemistry

Label

CoraFluor 1

Antibody Source

Monoclonal Mouse IgG2A Clone # 393314

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

HEK293 human embryonic kidney cell line transfected with human Claudin-12
Met1-Thr244
Accession # NP_036261

Specificity

Detects human Claudin-12. Stains human Claudin-12 transfectants but not irrelevant transfectants.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Description

CoraFluor(TM) 1 is a high performance terbium-based TR-FRET (Time-Resolved Fluorescence Resonance Energy Transfer) or TRF (Time-Resolved Fluorescence) donor for high throughput assay development. CoraFluor(IM) 1 absorbs UV light at approximately 340 nm, and emits at approximately 490 nm, 545 nm, 585 nm and 620 nm. It is compatible with common acceptor dyes that absorb at the emission wavelengths of CoraFluor(TM) 1. CoraFluor(TM) 1 can be used for the development of robust and scalable TR-FRET binding assays such as target engagement, ternary complex, protein-protein interaction and protein quantification assays.

Applications for Claudin-12 Antibody (393314) [CoraFluor™ 1]

Application
Recommended Usage

CyTOF-ready

Optimal dilutions of this antibody should be experimentally determined.

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

PBS

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark. Do not freeze.

Background: Claudin-12

Claudins, such as CLDN12, are components of epithelial cell tight junctions. Tight junctions regulate movement ofsolutes and ions through the paracellular space and prevent mixing of proteins and lipids in the outer leaflet of theapical and basolateral plasma membrane domains (Acharya et al., 2004).(supplied by OMIM)

Alternate Names

Claudin12, CLDN12

Gene Symbol

CLDN12

Additional Claudin-12 Products

Product Documents for Claudin-12 Antibody (393314) [CoraFluor™ 1]

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Claudin-12 Antibody (393314) [CoraFluor™ 1]

CoraFluor (TM) is a trademark of Bio-Techne Corp. Sold for research purposes only under agreement from Massachusetts General Hospital. US patent 2022/0025254

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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