Human Claudin-12 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB4284
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human
Applications
CyTOF-ready, Flow Cytometry, Immunocytochemistry
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2A Clone # 393314
Product Specifications
Immunogen
HEK293 human embryonic kidney cell line transfected with human Claudin-12
Met1-Thr244
Accession # NP_036261
Met1-Thr244
Accession # NP_036261
Specificity
Detects human Claudin-12. Stains human Claudin-12 transfectants but not irrelevant transfectants.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2A
Scientific Data Images for Human Claudin-12 Antibody
Detection of Claudin‑12 in Caco‑2 Human Cell Line
Caco-2 human colorectal adenocarcinoma cell line was stained with Mouse Anti-Human Claudin-12 Monoclonal Antibody (Catalog # MAB4284, filled histogram) or isotype control antibody (Catalog # MAB003, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG F(ab')2Secondary Antibody (Catalog # F0102B).
Claudin‑12 in NS0 Mouse Cell Line transfected with human Claudin-12
Claudin-12 was detected in immersion fixed NS0 mouse myeloma cell line transfected with human Claudin 12 using 10 µg/mL Mouse Anti-Human Claudin-12 Monoclonal Antibody (Catalog # MAB4284) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.Applications for Human Claudin-12 Antibody
Application
Recommended Usage
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Flow Cytometry
2.5 µg/106 cells
Sample: Caco‑2 human colorectal adenocarcinoma cell line
Sample: Caco‑2 human colorectal adenocarcinoma cell line
Immunocytochemistry
8-25 µg/mL
Sample: Immersion fixed NS0 mouse myeloma cell line transfected with human Claudin 12
Sample: Immersion fixed NS0 mouse myeloma cell line transfected with human Claudin 12
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Claudin-12
Claudin‑12 is a 244 amino acid multipass membrane protein in the claudin family of epithelial tight junction proteins. Claudin‑12 is expressed on intestinal, bladder, and blood‑brain barrier epithelial cells. Human Claudin‑12 shares 92% aa sequence identity with mouse and rat Claudin‑12.
Alternate Names
Claudin12, CLDN12
Gene Symbol
CLDN12
UniProt
Additional Claudin-12 Products
Product Documents for Human Claudin-12 Antibody
Product Specific Notices for Human Claudin-12 Antibody
For research use only
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