CXCR2/IL-8RB Antibody (547)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-43810

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Mouse

Predicted:

Chimpanzee (100%). Backed by our 100% Guarantee.

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # 547

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all CXCR2/IL-8RB Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of human CXCR2/IL-8RB. The exact sequence is proprietary.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Theoretical MW

41 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for CXCR2/IL-8RB Antibody (547)

Western Blot: CXCR2/IL-8RB Antibody (547) [NBP2-43810]

Western Blot: CXCR2/IL-8RB Antibody (547) [NBP2-43810]

Western Blot: CXCR2/IL-8RB Antibody (547) [NBP2-43810] - Non-transfected (-) and transfected (+) Boiled and unboiled 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with CXCR2 antibody [547] diluted at 1:5000. The HRP-conjugated anti-mouse IgG antibody (NBP2-19382) was used to detect the primary antibody.
Immunocytochemistry/ Immunofluorescence: CXCR2/IL-8RB Antibody (547) [NBP2-43810]

Immunocytochemistry/ Immunofluorescence: CXCR2/IL-8RB Antibody (547) [NBP2-43810]

Immunocytochemistry/Immunofluorescence: CXCR2/IL-8RB Antibody (547) [NBP2-43810] - CXCR2 antibody [547] detects CXCR2 protein by immunofluorescent analysis.Sample: Mock and transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: CXCR2 stained by CXCR2 antibody [547] diluted at 1:2000.Blue: Hoechst 33342 staining. Scale bar= 10um.
Western Blot: CXCR2/IL-8RB Antibody (547) [NBP2-43810]

Western Blot: CXCR2/IL-8RB Antibody (547) [NBP2-43810]

Western Blot: CXCR2/IL-8 RB Antibody (547) [NBP2-43810] - Analysis of Non-transfected (-) and CXCR2-transfected (+, including 3xFlag-tag) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with CXCR2 antibody diluted by 1:5000.

Applications for CXCR2/IL-8RB Antibody (547)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Western Blot

1:1000-1:10000

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH7), 20% Glycerol

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: CXCR2/IL-8RB

Chemokine (Chemoattractant Cytokines) are small peptides that are potent activators and chemo-attractants for leukocyte subpopulations and other non-hemopoietic cells. Chemokine receptors (CXCR) belong to the super-family of G protein-coupled receptors (GPCR), which regulate the trafficking and activation of leukocytes, and operate as co-receptors in the entry of HIV-1 and proliferation and migration of immature neurons, glia and their precursors. Furthermore, chemokine receptors participate in the etiology and progression of various brain disorders, including AIDS dementia, neuro-inflammatory disease and neuroplasia, making them important potential therapeutic targets in these cases several subtypes of CXCR have been characterized. Activation of na

Long Name

Interleukin 8 Receptor B

Alternate Names

CD182, CDw128b, CMKAR2, CXCR-2, IL-8 RB, IL8RB

Gene Symbol

CXCR2

Additional CXCR2/IL-8RB Products

Product Documents for CXCR2/IL-8RB Antibody (547)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for CXCR2/IL-8RB Antibody (547)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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