Cytokeratin 18 Antibody (C-04) - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NB500-306

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Validated:

Human, Mouse, Mammal

Cited:

Human, Mouse

Applications

Validated:

CyTOF-ready, ELISA, Flow (Intracellular), Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Cited:

ELISA, IF/IHC, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # C-04

Format

BSA Free

Concentration

1 mg/ml

View all Cytokeratin 18 Antibodies »

Product Specifications

Immunogen

Cytoskeleton preparation of epidermal carcinoma cell line A431.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Specificity

The antibody C-04 reacts with Cytokeratin 18 (45 kDa), a member of intermediate filaments subfamily represented in epithelial tissues.

Marker

Epithelial Marker

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

45 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Cytokeratin 18 Antibody (C-04) - BSA Free

Western Blot: Cytokeratin 18 Antibody (C-04)BSA Free [NB500-306]

Western Blot: Cytokeratin 18 Antibody (C-04)BSA Free [NB500-306]

Western Blot: Cytokeratin 18 Antibody (C-04) [NB500-306] - Analysis of cytokeratin 18 in HeLa cells with anti-cytokeratin 18 (C-04) biotin.
Immunohistochemistry-Paraffin: Cytokeratin 18 Antibody (C-04) - BSA Free [NB500-306]

Immunohistochemistry-Paraffin: Cytokeratin 18 Antibody (C-04) - BSA Free [NB500-306]

Immunohistochemistry-Paraffin: Cytokeratin 18 Antibody (C-04) [NB500-306] - Immunohistochemistry staining of human kidney (paraffin-embedded sections) with anti-cytokeratin 18 (C-04). Image using the Biotin format of this antibody.
Flow Cytometry: Cytokeratin 18 Antibody (C-04) - BSA Free [NB500-306]

Flow Cytometry: Cytokeratin 18 Antibody (C-04) - BSA Free [NB500-306]

Flow Cytometry: Cytokeratin 18 Antibody (C-04) [NB500-306] - Intracellular flow cytometry analysis of cytokeratin expression in HeLa human cervix carcinoma cell line using anti-cytokeratin 18 (C-04) FITC.
View 1 more images

Applications for Cytokeratin 18 Antibody (C-04) - BSA Free

Application
Recommended Usage

ELISA

1:2000

Flow (Intracellular)

1-4 ug/ml

Flow Cytometry

1-4 ug/ml

Immunocytochemistry/ Immunofluorescence

1:10-1:500

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Paraffin

10 ug/ml

Immunoprecipitation

1:50

Western Blot

1-2 ug/ml
Application Notes
Predicted molecular weight: 45 kDa. Western Blot - Resuspend approx. 50 mil. cells in 1 ml cold Lysis buffer (1% laurylmaltoside in 20 mM Tris/Cl, 100 mM NaCl pH 8.2, 50 mM NaF including Protease inhibitor Cocktail). Incubate 60 min on ice. Centrifuge to remove cell debris. Mix lysate with non-reducing SDS-PAGE sample buffer. Application note: Non-reducing conditions. SDS-PAGE (10% separating gel) ELISA - The antibody C-04 has been tested as the capture antibody in a sandwich ELISA for analysis of Cytokeratin 18 in combination with antibody DA-7 and with the antibody DC-10. This antibody is CyTOF ready.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH 7.4)

Format

BSA Free

Preservative

0.9% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: Cytokeratin 18

Cytokeratins are a subfamily of intermediate filaments and characterized by remarkable biochemical diversity. Cytokeratins are represented in epithelial tissues by at least 20 different polypeptides, molecular weight between 40 kDa and 68 kDa. The individual cytokeratin polypeptides are designated 1 to 20 and divided into the type I (acidic cytokeratins 9-20) and type II (basic to neutral cytokeratins 1-8) families. Cytokeratins 18 belongs to type I family (acidic cytokeratins).

Alternate Names

CK-18, CYK18, Keratin 18, KRT18

Gene Symbol

KRT18

Additional Cytokeratin 18 Products

Product Documents for Cytokeratin 18 Antibody (C-04) - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Cytokeratin 18 Antibody (C-04) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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