Cytokeratin 18 Antibody (C-04) [FITC]

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-48350

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Human, Mouse, Mammal

Applications

ELISA, Flow (Intracellular), Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Label

FITC (Excitation = 495 nm, Emission = 519 nm)

Antibody Source

Monoclonal Mouse IgG1 Clone # C-04

Concentration

0.1 mg/ml

View all Cytokeratin 18 Antibodies »

Product Specifications

Immunogen

Cytoskeleton preparation of epidermal carcinoma cell line A431.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Specificity

The antibody C-04 reacts with Cytokeratin 18 (45 kDa), a member of intermediate filaments subfamily represented in epithelial tissues.

Marker

Epithelial Marker

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

45 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Applications for Cytokeratin 18 Antibody (C-04) [FITC]

Application
Recommended Usage

ELISA

1:2000

Flow (Intracellular)

1-2 ug/ml

Flow Cytometry

1-2 ug/ml

Immunocytochemistry/ Immunofluorescence

1:10-1:500

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Paraffin

1:200

Immunoprecipitation

1:50

Western Blot

1:1000
Application Notes
Western blot, IHC and Flow Cytometry. IHC-P: Perform enzymatic antigen retrieval before commencing with IHC staining protocol. WB: Predicted molecular weight: 45 kDa. Optimal dilutions/concentrations should be determined by the end user.

Formulation, Preparation, and Storage

Purification

Size Exclusion Chromatography

Formulation

PBS and 0.2% (w/v) high grade BSA (protease free)

Preservative

0.9% Sodium Azide

Concentration

0.1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark.

Background: Cytokeratin 18

Cytokeratin 18 (CK-18), a normal constituent of the hepatocyte cytoskeleton) is a standard tumor marker. CK-18 is elevated in patients with non-malignant liver diseases, particularly in those with prominent cytolysis (1). CK-18 and the homologous mouse Endo B are type I IF protein subunits whose expression is restricted in adults to a variety of simple epithelial tissues. Keratin 8 and 18 (CK8/18) are the major keratin pair in simple-type epithelia, as found in the liver, pancreas, and intestine. Transgenic mice that express mutant keratin 18 develop chronic hepatitis, and have an increased susceptibility to drug-induced hepatotoxicity (2). Studies shows that high CK18 expression in tumor cells is associated with reduced invasiveness in vitro and lack of tumorigenicity in nude mice. It has been shown that high K18 expression correlated with a good prognosis and that reducing K18 expression increased the aggressiveness of established breast cancer cell lines (3).

Alternate Names

CK-18, CYK18, Keratin 18, KRT18

Gene Symbol

KRT18

Additional Cytokeratin 18 Products

Product Documents for Cytokeratin 18 Antibody (C-04) [FITC]

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for Cytokeratin 18 Antibody (C-04) [FITC]

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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