Cytokeratin 18 Antibody (C-04) [Biotin]
Novus Biologicals, part of Bio-Techne | Catalog # NB500-640
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Mammal
Applications
ELISA, Flow (Intracellular), Flow Cytometry, Immunocytochemistry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot
Label
Biotin
Antibody Source
Monoclonal Mouse IgG1 Clone # C-04
Concentration
1.0 mg/ml
Product Summary for Cytokeratin 18 Antibody (C-04) [Biotin]
Immunogen
Cytoskeleton preparation of epidermal carcinoma cell line A431.
Reactivity Notes
Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.
Specificity
The antibody C-04 reacts with Cytokeratin 18 (45 kDa), a member of intermediate filaments subfamily represented in epithelial tissues.
Marker
Epithelial Marker
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Theoretical MW
45 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for Cytokeratin 18 Antibody (C-04) [Biotin]
Western Blot: Cytokeratin 18 Antibody (C-04) [Biotin] [NB500-640] - Analysis of cytokeratin 18 in HeLa cells with anti-cytokeratin 18 (C-04) biotin.
Immunohistochemistry: Cytokeratin 18 Antibody (C-04) [Biotin] [NB500-640] - Immunohistochemistry staining of human colon (paraffin-embedded sections) with anti-cytokeratin 18 (C-04).
Flow Cytometry: Cytokeratin 18 Antibody (C-04) [Biotin] [NB500-640] - Intracellular flow cytometry analysis of cytokeratin expression in HeLa human cervix carcinoma cell line using anti-cytokeratin 18 (C-04) FITC. Overlay with Isotype mouse IgG1 control.
Applications for Cytokeratin 18 Antibody (C-04) [Biotin]
Application
Recommended Usage
ELISA
1:100-1:2000
Flow (Intracellular)
1-4 ug/ml
Flow Cytometry
1-4 ug/ml
Immunohistochemistry
10 ug/ml
Immunohistochemistry-Paraffin
10 ug/ml
Immunoprecipitation
1:10-1:500
Western Blot
1-2 ug/ml
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS (pH 7.4)
Preservative
0.9% Sodium Azide
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C. Do not freeze.
Background: Cytokeratin 18
Alternate Names
CK-18, CYK18, Keratin 18, KRT18
Gene Symbol
KRT18
Additional Cytokeratin 18 Products
Product Documents for Cytokeratin 18 Antibody (C-04) [Biotin]
Product Specific Notices for Cytokeratin 18 Antibody (C-04) [Biotin]
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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