Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Peptide ELISA, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat

Format

BSA Free

Concentration

1.18 mg/ml

View all ENPP-1 Antibodies »

Product Specifications

Immunogen

A synthetic peptide made to a C-terminal portion of the human ENPP1 protein (between residues 900-925) [UniProt P22413]

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (85%) and Rat (85%).

Localization

Membrane.

Clonality

Polyclonal

Host

Goat

Theoretical MW

140 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for ENPP-1 Antibody - BSA Free

Western Blot: ENPP-1 AntibodyBSA Free [NB600-816]

Western Blot: ENPP-1 AntibodyBSA Free [NB600-816]

Western Blot: ENPP-1 Antibody [NB600-816] - Western blot analysis of ENPP-1 in HepG2 cell lysate at 2.0 ug/ml.
Immunohistochemistry-Paraffin: ENPP-1 Antibody - BSA Free [NB600-816]

Immunohistochemistry-Paraffin: ENPP-1 Antibody - BSA Free [NB600-816]

Immunohistochemistry-Paraffin: ENPP-1 Antibody [NB600-816] - ENPP1 Antibody [NB600-816] - ENPP1 antibody was tested in human liver using DAB with hematoxylin counterstain.

Applications for ENPP-1 Antibody - BSA Free

Application
Recommended Usage

Immunohistochemistry-Paraffin

1:200

Peptide ELISA

1:2000

Western Blot

1:1000
Application Notes
This ENPP1 antibody is useful for Peptide ELISA, Western Blot and Immunohistochemistry-paraffin embedded sections. In Western blot a band was observed at ~130-150kDa in Human Liver and HepG2 lysates that was blocked by preincubation with the immunizing peptide. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS and 30% Glycerol

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

1.18 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: ENPP-1

ENPP1 (ectonucleotide pyrophosphatase/phosphodiesterase family member 1) is a type II transmembrane glycoprotein which is implicated in membrane surface ATP hydrolysis, regulation of pyrophosphate levels, bone mineralization as well as soft tissue calcification. ENPP1 negatively regulates bone mineralization by hydrolyzing extracellular nucleotide triphosphates (NTPs) to produce inorganic pyrophosphate (PPi, an inhibitor of mineralization), whereas tissue-nonspecific ALP positively regulates mineralization by hydrolyzing NTPs and PPi to produce extracellular concentrations of inorganic phosphate (Pi, a substrate for mineralization). ENPP1 is expressed on outer surfaces of mineralizing cells, such as osteoblasts and chondrocytes, and on the membranes of osteoblast- and chondrocyte-derived matrix vesicles. It also hydrolyzes other NTPs such as GTP, CTP, TTP, UTP etc. to their corresponding monophosphates with the release of PPi and diadenosine polyphosphates, and also 3',5'-cAMP to AMP, and may also regulate the availability of nucleotide sugars in ER/Golgi complex, as well as the regulation of purinergic signaling. ENPP1 binds directly to INSR for inhibiting insulin signaling and ENPP1's K173Q/ K121Q polymorphism is associated with insulin resistance, NIDDM, and obesity. Defective ENPP1 causes increased susceptibility for ossification of the posterior longitudinal ligament of spine (OPLL), arterial calcification of infancy, generalized, type 1 (GACI1), obesity/glucose intolerance/NIDDM, and rickets hypophosphatemic autosomal recessive type 2 (ARHR2).

Long Name

Ectonucleotide Pyrophosphatase/Phosphodiesterase 1

Alternate Names

ENPP1, Ly-41 Antigen, M6S1, NPP1, NPPS, PCA1, PDNP1

Entrez Gene IDs

5167 (Human)

Gene Symbol

ENPP1

UniProt

P22413 (Human)

Additional ENPP-1 Products

Product Documents for ENPP-1 Antibody - BSA Free

Product Datasheets

Certificate of Analysis

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Product Specific Notices for ENPP-1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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